Document Detail


Presence of functional type B natriuretic peptide receptor in human ocular cells.
MedLine Citation:
PMID:  8759339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To study the effects of natriuretic peptides on cyclic guanosine monophosphate (cGMP) production and calcium mobilization in cultured human ocular cells. METHODS: Cultured simian virus 40-transformed (HTM-3) and nontransformed (HTM-16) human trabecular meshwork (TM) cells and nontransformed human ciliary muscle (CM) cells were used. Accumulation of cGMP in cells lysate was measured by radioimmunoassay. Intracellular calcium concentration was measured by microscope-based ratiofluorometry. RESULTS: Both atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP) increased the accumulation of cGMP in HTM-3, HTM-16, and CM cells. In the nontransformed TM cells, CNP was five times more efficacious (maximal effect of CNP was 497% +/- 44% that of ANP) and 10 times more potent than ANP (ANP, log [EC50] = -6.99 +/- 0.08; CNP, log [EC50] = -7.96 +/- 0.20). Similar results were seen in HTM-3 and CM cells. Under the assay conditions used, the peptides increased only the production of cGMP without changing its degradation rate. The peptide-induced increase of cGMP in the TM and CM cells correlated with suppression of carbachol-induced calcium mobilization in the cell. CONCLUSIONS: It is known that CNP, but not ANP, selectively activates the guanylyl cyclase associated with the type B natriuretic peptide receptor (NPR-B). Thus, the data suggest that NPR-B is the primary functional NPR in the TM and CM cells. The effects on cGMP and calcium produced by the activation of this receptor are expected to alter TM and CM contractility and may affect aqueous humor hydrodynamics and intraocular pressure.
Authors:
I H Pang; D L Shade; S Matsumoto; H T Steely; L DeSantis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  37     ISSN:  0146-0404     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1996-09-18     Completed Date:  1996-09-18     Revised Date:  2010-03-24    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1724-31     Citation Subset:  IM    
Affiliation:
Alcon Laboratories, University of North Texas Health Science Center, Fort Worth, USA.
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MeSH Terms
Descriptor/Qualifier:
1-Methyl-3-isobutylxanthine / pharmacology
Atrial Natriuretic Factor / pharmacology*
Calcium / metabolism*
Carbachol / pharmacology
Cell Line, Transformed
Cells, Cultured
Ciliary Body / drug effects,  metabolism,  physiology*
Cyclic GMP / metabolism*
Guanylate Cyclase / drug effects,  metabolism,  physiology*
Humans
Intraocular Pressure
Kinetics
Muscle Contraction
Natriuretic Peptide, C-Type
Proteins / pharmacology*
Receptors, Atrial Natriuretic Factor / drug effects,  physiology*
Simian virus 40
Trabecular Meshwork / drug effects,  metabolism,  physiology*
Chemical
Reg. No./Substance:
0/Proteins; 127869-51-6/Natriuretic Peptide, C-Type; 28822-58-4/1-Methyl-3-isobutylxanthine; 51-83-2/Carbachol; 7440-70-2/Calcium; 7665-99-8/Cyclic GMP; 85637-73-6/Atrial Natriuretic Factor; EC 4.6.1.2/Guanylate Cyclase; EC 4.6.1.2/Receptors, Atrial Natriuretic Factor; EC 4.6.1.2/atrial natriuretic factor receptor C

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