| Presence of functional, autoreactive human milk-specific IgE in infants with cow's milk allergy. | |
| | |
MedLine Citation:
|
PMID: 22092935 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Occasionally, exclusively breastfed infants with cow's milk allergy (CMA) remain symptomatic despite strict maternal milk avoidance. OBJECTIVE: To determine whether or not persistence of symptoms could be due to sensitization against endogenous human milk proteins with a high degree of similarity to bovine allergens. METHODS: Ten peptides representing known bovine milk IgE-binding epitopes [α-lactalbumin (ALA), β- and κ-casein] and the corresponding, highly homologous human milk peptides were labelled with sera from 15 breastfed infants with CMA, aged 3 weeks to 12 months, and peptide (epitope)-specific IgE antibodies were assessed. Nine of the 15 breastfed infants became asymptomatic during strict maternal avoidance of milk and other major food allergens; six infants remained symptomatic until weaned. Ten older children, aged 5-15 years, with CMA were also assessed. The functional capacity of specific IgE antibodies was assessed by measuring β-hexosaminidase release from rat basophilic leukaemia cells passively sensitized and stimulated with human and bovine ALA. RESULTS: A minimum of one human milk peptide was recognized by IgE antibodies from 9 of 15 (60%) milk-allergic infants, and the majority of older children with CMA. Genuine sensitization to human milk peptides in the absence of IgE to bovine milk was occasionally seen. There was a trend towards specific IgE being detected to more human milk peptides in those infants who did not respond to the maternal milk elimination diet than in those who did (P = 0.099). Functional IgE antibody to human ALA was only detected in infants not responding to the maternal diet. CONCLUSIONS AND CLINICAL RELEVANCE: Endogenous human milk epitopes are recognized by specific IgE from the majority of infants and children with CMA. Such autoreactive, human milk-specific IgE antibodies appear to have functional properties in vitro. Their role in provoking allergic symptoms in infants exclusively breastfed by mothers strictly avoiding dietary milk remains unclear. |
| | |
Authors:
|
K M Järvinen; L Geller; R Bencharitiwong; H A Sampson |
Related Documents
:
|
21989065 - Where's waldo? the 'decapitation gambit' and the definition of death. 20098005 - Occult enterorrhagia from jejunal duplication causing diagnostic dilemma in an infant. 22247245 - A proposed new international convention supporting the rights of pregnant women and gir... 16641995 - Heterotrophic plasticity and resilience in bleached corals. 995015 - Intraventricular haemorrhage in the newborn. 10984575 - Racial and ethnic variation in low birthweight in the united states: individual and con... |
Publication Detail:
|
Type: Clinical Trial; Journal Article; Research Support, N.I.H., Extramural Date: 2011-09-21 |
Journal Detail:
|
Title: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology Volume: 42 ISSN: 1365-2222 ISO Abbreviation: Clin. Exp. Allergy Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2012-01-31 Completed Date: 2012-05-22 Revised Date: 2013-01-31 |
Medline Journal Info:
|
Nlm Unique ID: 8906443 Medline TA: Clin Exp Allergy Country: England |
Other Details:
|
Languages: eng Pagination: 238-47 Citation Subset: IM |
Copyright Information:
|
© 2011 Blackwell Publishing Ltd. |
Affiliation:
|
Division of Pediatric Allergy & Immunology, The Mount Sinai School of Medicine, New York, NY 10029-6574, USA. kirsi.jarvinen-seppo@mssm.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antibody Specificity / genetics, immunology* Breast Feeding Cattle Cell Line, Tumor Child Child, Preschool Female Humans Immunoglobulin E / blood, genetics, immunology* Infant Infant, Newborn Male Milk Hypersensitivity / blood, genetics, immunology* Milk Proteins / genetics, immunology* Peptides / genetics, immunology* Rats Sequence Homology, Amino Acid |
| Grant Support | |
ID/Acronym/Agency:
|
AI066738/AI/NIAID NIH HHS; AI44236/AI/NIAID NIH HHS; K08 AI091655/AI/NIAID NIH HHS; K12 HD052890-03/HD/NICHD NIH HHS; RR026134/RR/NCRR NIH HHS; UL1 RR029887/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Milk Proteins; 0/Peptides; 37341-29-0/Immunoglobulin E |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The relation between coronary artery calcification in asymptomatic subjects and both traditional ris...
Next Document: Alkaline Fe(III) reduction by a novel alkali tolerant Serratia Sp. isolated from surface sediments c...