| Presence of epsilon-adenosine tetraphosphate in chromaffin granules after transport of epsilon-ATP. | |
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MedLine Citation:
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PMID: 8706915 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adenosine 5'-tetraphosphate (Ap4) is a natural constituent of chromaffin granules with concentration values of 2.2 +/- 0.1 nmol/mg of protein and a ratio 245 +/- 40 times lower with respect to ATP (n = 4). The granular transport of epsilon-ATP resulted in a time- and concentration-dependent production of epsilon-adenosine tetraphosphate (epsilon-Ap4) at the intragranular level. The epsilon-Ap4 formation followed a hyperbolic saturation kinetic at low epsilon-ATP concentrations with K(m) value of 0.4 microM epsilon-ATP intragranular (1.15 pmol/mg of granular protein). Intragranular concentrations of epsilon-ATP higher than 500 pmol/mg of protein (approximately to 175 microM intragranular) resulted in a non-saturable production of epsilon-Ap4. |
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Authors:
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J Gualix; M Abal; J Pintor; M T Miras-Portugal |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 391 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1996 Aug |
Date Detail:
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Created Date: 1996-09-12 Completed Date: 1996-09-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 195-8 Citation Subset: IM |
Affiliation:
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Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenine Nucleotides
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isolation & purification,
metabolism* Animals Biological Transport Chromaffin Granules / metabolism* Chromatography, High Pressure Liquid Ethenoadenosine Triphosphate / metabolism* Kinetics |
| Chemical | |
Reg. No./Substance:
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0/Adenine Nucleotides; 0/Ethenoadenosine Triphosphate; 1062-98-2/adenosine 5'-tetraphosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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