Document Detail

Presence of epsilon-adenosine tetraphosphate in chromaffin granules after transport of epsilon-ATP.
MedLine Citation:
PMID:  8706915     Owner:  NLM     Status:  MEDLINE    
Adenosine 5'-tetraphosphate (Ap4) is a natural constituent of chromaffin granules with concentration values of 2.2 +/- 0.1 nmol/mg of protein and a ratio 245 +/- 40 times lower with respect to ATP (n = 4). The granular transport of epsilon-ATP resulted in a time- and concentration-dependent production of epsilon-adenosine tetraphosphate (epsilon-Ap4) at the intragranular level. The epsilon-Ap4 formation followed a hyperbolic saturation kinetic at low epsilon-ATP concentrations with K(m) value of 0.4 microM epsilon-ATP intragranular (1.15 pmol/mg of granular protein). Intragranular concentrations of epsilon-ATP higher than 500 pmol/mg of protein (approximately to 175 microM intragranular) resulted in a non-saturable production of epsilon-Ap4.
J Gualix; M Abal; J Pintor; M T Miras-Portugal
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  391     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1996-09-12     Completed Date:  1996-09-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  195-8     Citation Subset:  IM    
Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
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MeSH Terms
Adenine Nucleotides / isolation & purification,  metabolism*
Biological Transport
Chromaffin Granules / metabolism*
Chromatography, High Pressure Liquid
Ethenoadenosine Triphosphate / metabolism*
Reg. No./Substance:
0/Adenine Nucleotides; 0/Ethenoadenosine Triphosphate; 1062-98-2/adenosine 5'-tetraphosphate

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