| Presence of di- and polyamines covalently bound to protein in rat liver. | |
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MedLine Citation:
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PMID: 2861856 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Acid hydrolysis of trichloroacetic acid precipitate from rat tissue (liver, kidney and testis) homogenate released significant amounts of acid-insoluble putrescine, spermidine and spermine. Following incubation of liver homogenate with [1,4-14C]putrescine, 1.4% of total radioactivity and 1.0% of labelled diamine were recovered in the acid-insoluble fraction. Exhaustive digestion of acid-precipitable material with proteinases (Pronase, aminopeptidase M, carboxypeptidase A, B and Y) revealed the presence of di- and polyamines and of N1-(gamma-glutamyl)spermidine, N1-(gamma-glutamyl)spermine and N1,N12-bis(gamma-glutamyl)spermine. These derivatives were identified both by chromatographic analysis and by enzymatic digestion with purified gamma-glutamylamine cyclotransferase. The finding of di- and polyamine gamma-glutamyl derivatives in the proteinase-digested acid-insoluble fraction of homogenate may be considered as a proof of the in vivo transglutaminase-catalyzed binding of polyamines to proteins. This evidence suggests that di- and polyamines might have an important role in mammalian tissues through covalent binding to proteins by either one or both the primary amino groups. |
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Authors:
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S Beninati; M Piacentini; M P Argento-Cerù; S Russo-Caia; F Autuori |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 841 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1985 Jul |
Date Detail:
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Created Date: 1985-09-06 Completed Date: 1985-09-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 120-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, Ion Exchange Glutamine / metabolism Kidney / metabolism Liver / analysis* Male Peptide Hydrolases / metabolism Polyamines / metabolism* Protein Processing, Post-Translational* Proteins / metabolism* Putrescine / metabolism Rats Rats, Inbred Strains Spermidine / metabolism Spermine / metabolism Testis / metabolism gamma-Glutamylcyclotransferase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Polyamines; 0/Proteins; 110-60-1/Putrescine; 124-20-9/Spermidine; 56-85-9/Glutamine; 71-44-3/Spermine; EC 2.3.2.4/gamma-Glutamylcyclotransferase; EC 3.4.-/Peptide Hydrolases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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