Document Detail


Presence of the complement-regulatory protein membrane cofactor protein (MCP, CD46) as a membrane-associated product in seminal plasma.
MedLine Citation:
PMID:  7861396     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The seminal plasma complement regulator membrane cofactor protein (MCP) was examined by sequential centrifugation and phase separation in the detergent Triton X-114. The presence of MCP components in seminal plasma depleted of the 40 kDa sperm MCP product by low speed centrifugation was confirmed. Subsequent centrifugation at 2200 g recovered a pellet containing a prominent 60 kDa and a weak 50-55 kDa MCP component. A 60 kDa MCP product remained detectable in the supernatant fraction after this centrifugation step but this was depleted by ultracentrifugation. Recovery of the seminal plasma MCP components in the pellet fraction obtained by ultracentrifugation suggested that seminal plasma MCP is membrane-associated. Seminal plasma fractions were also subjected to phase separation in Triton X-114. MCP components in both pellet and supernatant fractions partitioned to the detergent phase, confirming that seminal plasma MCP is membrane associated. The origin of these proteins was investigated by analysing MCP products in seminal plasma from vasectomized men. The 40 kDa sperm MCP protein was absent but a 60 kDa MCP component, which partitioned to the detergent phase in Triton X-114, was evident. Seminal plasma therefore contains typical membrane-associated MCP products that appear to be derived distal to the ductus deferens.
Authors:
K L Simpson; C H Holmes
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  102     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1995-03-23     Completed Date:  1995-03-23     Revised Date:  2010-08-25    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  419-24     Citation Subset:  IM    
Affiliation:
University of Bristol, Department of Obstetrics and Gynaecology, St Michael's Hospital, UK.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / analysis*
Antigens, CD46
Cell Membrane / immunology
Centrifugation
Complement System Proteins / immunology*
Detergents
Humans
Immunoblotting
Male
Membrane Glycoproteins / analysis*
Methods
Polyethylene Glycols
Semen / immunology*
Vasectomy
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD46; 0/CD46 protein, human; 0/Detergents; 0/Membrane Glycoproteins; 0/Polyethylene Glycols; 9007-36-7/Complement System Proteins; 9036-19-5/Nonidet P-40

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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