Document Detail

Presence of cellular renin-angiotensin system in chromaffin cells of bovine adrenal medulla.
MedLine Citation:
PMID:  12384458     Owner:  NLM     Status:  MEDLINE    
The presence of a local renin-angiotensin system has been established in organs that serve as angiotensin targets. In this study, the expression of angiotensinogen mRNA and subcellular localization of renin, angiotensin-converting enzyme, and angiotensin II were investigated in bovine adrenal medullary cells in primary culture. By light microscopy, expression of angiotensinogen mRNA, immunoreactive renin, angiotensin-converting enzyme, and angiotensin II were readily detectable only in the chromaffin cells. The density distribution of renin and angiotensin II in sucrose gradients suggested a concentration in chromaffin granules, a localization directly confirmed by immunoelectron microscopy. Reverse transcriptase-polymerase chain reaction and sequencing confirmed the expression of angiotensinogen in bovine chromaffin cells and the adrenal medulla. In addition, in vitro autoradiography indicated that both angiotensin-converting enzyme and angiotensin type 1 receptors were present in the adrenal medulla. These results provide the first direct evidence that chromaffin cells in the adrenal medulla are not only the target for angiotensin but should also be considered as potential local angiotensin-generating and -storing cells.
Jun Ming Wang; Dirk Slembrouck; Junhui Tan; Lut Arckens; Frans H H Leenen; Pierre J Courtoy; Werner P De Potter
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  283     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-10-17     Completed Date:  2002-11-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H1811-8     Citation Subset:  IM    
Laboratory of Neuropharmacology and Neurobiology, Department of Medicine, Universitaire Instelling Antwerpen, University of Antwerp, B-2610 Antwerp, Belgium.
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MeSH Terms
Adrenal Medulla / cytology*
Angiotensin II / analysis
Angiotensinogen / genetics
Cells, Cultured
Centrifugation, Isopycnic
Chromaffin Cells / chemistry,  physiology*,  ultrastructure
In Situ Hybridization
Microscopy, Immunoelectron
Peptidyl-Dipeptidase A / analysis
RNA, Messenger / analysis
Radioligand Assay
Receptor, Angiotensin, Type 1
Receptors, Angiotensin / analysis
Renin / analysis
Renin-Angiotensin System / physiology*
Reg. No./Substance:
0/RNA, Messenger; 0/Receptor, Angiotensin, Type 1; 0/Receptors, Angiotensin; 11002-13-4/Angiotensinogen; 11128-99-7/Angiotensin II; 57-50-1/Sucrose; EC A; EC

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