Document Detail


Presence and antibiotic resistance of Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens in children.
MedLine Citation:
PMID:  12445225     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIMS: Only limited information exists about the prevalence in children of pathogens associated with periodontitis. The aim of the present study was to determine by culture whether 8-11-year-old children carry Porphyromonas gingivalis, Prevotella intermedia, and/or P. nigrescens in samples from the gingiva and/or the buccal mucosa taken before, and after caries treatment and oral hygiene instruction. A second aim was to assess the proportion of subjects who had gram-negative anaerobes carrying the tet(Q) and erm(F) genes, suggesting antibiotic resistance to tetracycline or erythromycin. METHOD: A total of 150 children provided gingival and buccal swab bacterial samples that were cultured for P. gingivalis, P. intermedia, and P. nigrescens. The species was verified using DNA-DNA hybridization with species-specific probes made from the variable region of the 16S rRNA sequences. Antibiotic-resistant genes, tet(Q) and erm(F), were identified using specific DNA-DNA hybridization with specific DNA probes. RESULTS: A total of 116 isolates of black-pigmented bacteria were cultured from 47 (31%) of 150 children. Five isolates were identified as P. gingivalis, 29 as P. intermedia, 33 as P. nigrescens, and 49 as other species. In general, the bacteria were not culturable at more than one time period. We found that 55% of these 47 children harbored black pigmented bacteria that carried either one or both of the two antibiotic-resistant genes studied (tet(Q), and erm(F)). CONCLUSION: The present study demonstrated that children not exposed to regular dental treatment carry bacteria outside the gingival sulcus that have been associated with periodontitis, and that standard treatment procedures may not clear the presence of the putative pathogens. In addition, antibiotic-resistant genes are common in identifiable gram-negative anaerobes, including putative pathogens.
Authors:
Yasaman Sanai; G Rutger Persson; Jacqueline R Starr; Henrique S Luis; Mario Bernardo; Jorge Leitao; Marilyn C Roberts
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of clinical periodontology     Volume:  29     ISSN:  0303-6979     ISO Abbreviation:  J. Clin. Periodontol.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-11-26     Completed Date:  2003-03-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0425123     Medline TA:  J Clin Periodontol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  929-34     Citation Subset:  D; IM    
Affiliation:
Department of Periodontics, University of Washington, Seattle, Washington 98195, USA.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Bacterial Typing Techniques
Child
Colony Count, Microbial
DNA, Bacterial / analysis
Dental Plaque / microbiology*
Drug Resistance, Bacterial / genetics*
Female
Genes, Bacterial
Humans
Male
Nucleic Acid Hybridization
Porphyromonas gingivalis / drug effects,  genetics*,  isolation & purification*
Prevotella / drug effects,  genetics*,  isolation & purification*
Statistics, Nonparametric
Grant Support
ID/Acronym/Agency:
1894//PHS HHS; DE-72623/DE/NIDCR NIH HHS; T32 DE07227/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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