| Presence of anti-insulin natural autoantibodies in healthy cats and its interference with immunoassay for serum insulin concentrations. | |
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MedLine Citation:
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PMID: 19850439 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A substance interfering with the enzyme-linked immunosorbent assay (ELISA) for feline insulin concentration was investigated in healthy cats. An insulin-binding substance isolated from feline serum showed 2 bands at 25 and 50 kDa in SDS-PAGE, suggesting the presence of immunoglobulin G (IgG). Insulin-binding IgG from healthy cats indeed reduced insulin immunoreactivity in the ELISA for determining insulin concentration. The insulin-binding IgG was polyclonal/polyreactive and showed certain specificity, high affinity, and high binding capacity, which was evaluated by liquid-phase radioimmunoassay with Scatchard plot analysis. Epitope analysis revealed that the insulin-binding IgG showed significant binding at residues A1-5 and B20-30 of the insulin molecule. Removal of the antibodies from serum enabled the determination of serum insulin concentrations by ELISA. Our data indicated that serum from healthy cats contained substantial amounts of natural autoantibodies combined with insulin, and that the antibodies interfered with the heterologous immunoassay for serum insulin concentration. |
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Authors:
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N Nishii; M Takasu; M Kojima; T Hachisu; K Wakabayashi; A Iwasawa; S Maeda; Y Ohba; H Kitagawa |
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Publication Detail:
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Type: Journal Article Date: 2009-10-21 |
Journal Detail:
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Title: Domestic animal endocrinology Volume: 38 ISSN: 1879-0054 ISO Abbreviation: Domest. Anim. Endocrinol. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-02 Completed Date: 2010-08-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8505191 Medline TA: Domest Anim Endocrinol Country: United States |
Other Details:
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Languages: eng Pagination: 138-45 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier Inc. All rights reserved. |
Affiliation:
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Laboratory of Veterinary Pharmacotherapeutics, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoantibodies / blood*, immunology Cats Cross Reactions Enzyme-Linked Immunosorbent Assay* Epitope Mapping Epitopes Immunoglobulin G Insulin / blood*, immunology Insulin Antibodies / blood*, immunology Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Autoantibodies; 0/Epitopes; 0/Immunoglobulin G; 0/Insulin Antibodies; 11061-68-0/Insulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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