Document Detail

The presence of P-glycoprotein in L1210 cells directly induces down-regulation of cell surface saccharide targets of concanavalin A.
MedLine Citation:
PMID:  20944151     Owner:  NLM     Status:  MEDLINE    
Overexpression of P-glycoprotein (P-gp), a plasma membrane drug transporter (ABCB1, a member of the ABC transporter family), is the most prevalent cause of multidrug resistance in cancer tissues. Lectin concanavalin A (ConA) induces massive cell death of L1210 leukemia cells (S). Cell sublines of L1210 in which P-gp overexpression was induced by selection with vincristine (R) or by stable transfection with a plasmid encoding full-length human P-gp (T) were less sensitive to ConA. Both P-gp-positive cell lines exhibited typical P-gp-mediated multidrug resistance. Resistance of R and T cells to ConA was associated with lower binding of ConA as compared to S cells when analysed by the following methods: (i) SDS PAGE and electroblotting of proteins in the crude membrane fraction followed by detection with biotinylated ConA and avidin-peroxidase, and (ii) fluorescent cytometry or confocal microscopy of the intact cells with surfaces labeled by FITC-ConA. These data indicated that the presence of P-glycoprotein in L1210 cells independently of the mode of its expression induced down-regulation of cell surface saccharide targets of ConA. Therefore, this feature may be considered as a secondary cellular response to P-glycoprotein expression.
Zdena Sulová; Peter Ditte; Tatiana Kurucová; Eva Poláková; Kristína Rogozanová; Lenka Gibalová; Mário Seres; Lucia Skvarková; Ján Sedlák; Jaromír Pastorek; Albert Breier
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-10-14     Completed Date:  2010-11-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  3661-8     Citation Subset:  IM    
Institute of Molecular Physiology and Genetics, Centre of Excellence of the Slovak Research and Development Agency Biomembranes2008, Slovak Academy of Sciences, 83334 Bratislava, Slovak Republic.
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MeSH Terms
Cell Line, Tumor
Cell Membrane / metabolism*
Cell Separation
Concanavalin A / metabolism*
Drug Resistance, Neoplasm*
Electrophoresis, Polyacrylamide Gel
Flow Cytometry
Glycoproteins / metabolism
Microscopy, Confocal
P-Glycoprotein / metabolism*
Reg. No./Substance:
0/Glycoproteins; 0/P-Glycoprotein; 11028-71-0/Concanavalin A

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