Document Detail


Presence of MRP8 and MRP14 in pancreatic cell lines: differential expression and localization in CFPAC-1 cells.
MedLine Citation:
PMID:  7762618     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A complex of two calcium binding proteins, MRP8 [also called cystic fibrosis (CF) antigen] and MRP14, proteins known to be expressed in cells of myeloid origin, has been shown to be present in higher amounts in the serum of CF patients and heterozygotes compared with normal subjects. We demonstrated here for the first time, by dot-blot analysis and immunocytochemistry, the expression and the presence of these S100 calcium binding proteins in the pancreatic cell lines CAPAN-1 and CFPAC-1, the latter provided from a patient with CF. Moreover, using immunocytochemical methods, we showed that the localization of MRP8 and MRP14 on the plasma membrane seems to be restricted to the cells expressing a cystic fibrosis transmembrane conductance regulator (CFTR) wild-type protein such as CAPAN-1 cells and CFPAC-1 cells transfected with a plasmid containing the nonmutated CFTR gene (CFPAC-PLJ-CFTR-6 cells). In CFPAC-1 cells, immunoreactivity remains in the cytoplasm throughout the stationary phase. We also showed an increased level of the mRNAs of the two proteins in the CFPAC-1 cells compared with those transfected with the nonmutated CFTR. The demonstration of a difference in the cellular localization of these two proteins and in their mRNA levels in the cell line of CF origin leads us to assume the existence of a possible correlation in the expression of the MRPs with that of the CFTR protein.
Authors:
M Fanjul; W Renaud; M Merten; O Guy-Crotte; E Hollande; C Figarella
Related Documents :
22342628 - β-carboline alkaloids from the leaves of trigonostemon lii y.t. chang.
19967048 - Role of caveolin-1 in indomethacin-induced death of human hepatoadenocarcinoma sk-hep1 ...
15782208 - Localized transfection on arrays of magnetic beads coated with pcr products.
11300748 - High-efficiency non-viral transfection of primary chondrocytes and perichondrial cells ...
17408918 - Effects of benzo(e)pyrene and benzo(a)pyrene on p-glycoprotein-mediated transport in ca...
14283628 - The ultrastructure of the kinocilium of the sensory cells in the inner ear and lateral ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  268     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-06-29     Completed Date:  1995-06-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C1241-51     Citation Subset:  IM    
Affiliation:
Laboratoire de Biologie Cellulaire, Université Paul Sabatier, Toulouse, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antigens, Differentiation / genetics,  metabolism*
Calcium-Binding Proteins / genetics,  metabolism*
Calgranulin A
Calgranulin B
Cell Line
Cell Membrane / metabolism
Cystic Fibrosis / metabolism*,  pathology
Humans
Immunoblotting
Immunohistochemistry
Microscopy, Electron
Pancreas / metabolism*,  pathology
RNA, Messenger / metabolism
Tissue Distribution
Chemical
Reg. No./Substance:
0/Antigens, Differentiation; 0/Calcium-Binding Proteins; 0/Calgranulin A; 0/Calgranulin B; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Cyclic ADP-ribose and related compounds activate sheep skeletal sarcoplasmic reticulum Ca2+ release ...
Next Document:  Stimulation of Na(+)-K(+)-ATPase by thyrotropin in cultured thyroid follicular cells.