Document Detail


Presence of IL-1- and TNF-like molecules in Galleria mellonella (Lepidoptera) haemocytes and in an insect cell line Fromestigmene acraea (Lepidoptera).
MedLine Citation:
PMID:  10479399     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study the authors give immunocytochemical evidence for the presence of interleukin (IL)-1alpha- and tumour necrosis factor (TNF) alpha-like molecules in the haemocytes of last instar larvae from the greater wax moth Galleria mellonella. Similar results are demonstrated in a continuous haemocyte line (BTI-EA-1174-A) from the salt marsh caterpillar Estigmene acraea. In Galleria mellonella larvae granular cells show a strong positive reaction with both primary antibodies, whereas plasmatocytes are stained to a lesser extent. Cell line haemocytes also react positively with both antibodies. After activating the cells with lipopolysaccharide (LPS) staining of Estigmene acraea cells is decreased, whereas Galleria mellonella haemocytes show no visible reaction in comparison to non-activated cells.
Authors:
D Wittwer; A Franchini; E Ottaviani; A Wiesner
Related Documents :
11853689 - Methionine synthase activity and sulphur amino acid levels in the rat liver tumour cell...
6846019 - Electro-physiological measurements in cultured cellular spheroids.
12835669 - Molecular mechanisms of glioma invasiveness: the role of proteases.
25198659 - Activation of upar is required for cigarette smoke extract-induced epithelial-mesenchym...
1165439 - Calcitonin production by rat thyroid tumours.
12861379 - Initiation of genetic instability and tumour formation: a review and hypothesis of a no...
2538229 - Characterization of hamster tumors induced by simian virus 40 small t deletion mutants ...
20493909 - Morgana/chp-1 is a novel chaperone able to protect cells from stress.
17084479 - Glycated collagen i (gc) impairs angiogenesis in vitro: a study using an innovative cha...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytokine     Volume:  11     ISSN:  1043-4666     ISO Abbreviation:  Cytokine     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-10-21     Completed Date:  1999-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9005353     Medline TA:  Cytokine     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  637-42     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Affiliation:
Institut für Zoologie, Freie Universität Berlin, Berlin, 14195, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antibody Specificity
Cell Line
Evolution, Molecular
Hemocytes / chemistry*
Hemolymph / chemistry*
Immunoenzyme Techniques
Interleukin-1 / blood*
Larva
Lepidoptera / chemistry*,  growth & development
Tumor Necrosis Factor-alpha / analysis*
Chemical
Reg. No./Substance:
0/Interleukin-1; 0/Tumor Necrosis Factor-alpha

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Preschoolers' and adults' reliance on object shape and object function for lexical extension.
Next Document:  Distinct roles for p42/p44 and p38 mitogen-activated protein kinases in the induction of IL-2 by IL-...