Document Detail

Presence of IL-1- and TNF-like molecules in Galleria mellonella (Lepidoptera) haemocytes and in an insect cell line Fromestigmene acraea (Lepidoptera).
MedLine Citation:
PMID:  10479399     Owner:  NLM     Status:  MEDLINE    
In this study the authors give immunocytochemical evidence for the presence of interleukin (IL)-1alpha- and tumour necrosis factor (TNF) alpha-like molecules in the haemocytes of last instar larvae from the greater wax moth Galleria mellonella. Similar results are demonstrated in a continuous haemocyte line (BTI-EA-1174-A) from the salt marsh caterpillar Estigmene acraea. In Galleria mellonella larvae granular cells show a strong positive reaction with both primary antibodies, whereas plasmatocytes are stained to a lesser extent. Cell line haemocytes also react positively with both antibodies. After activating the cells with lipopolysaccharide (LPS) staining of Estigmene acraea cells is decreased, whereas Galleria mellonella haemocytes show no visible reaction in comparison to non-activated cells.
D Wittwer; A Franchini; E Ottaviani; A Wiesner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytokine     Volume:  11     ISSN:  1043-4666     ISO Abbreviation:  Cytokine     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-10-21     Completed Date:  1999-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9005353     Medline TA:  Cytokine     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  637-42     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Institut für Zoologie, Freie Universität Berlin, Berlin, 14195, Germany.
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MeSH Terms
Antibody Specificity
Cell Line
Evolution, Molecular
Hemocytes / chemistry*
Hemolymph / chemistry*
Immunoenzyme Techniques
Interleukin-1 / blood*
Lepidoptera / chemistry*,  growth & development
Tumor Necrosis Factor-alpha / analysis*
Reg. No./Substance:
0/Interleukin-1; 0/Tumor Necrosis Factor-alpha

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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