Document Detail

The presence of histidine-rich glycoprotein in the female reproductive tract and in embryos.
MedLine Citation:
PMID:  20639474     Owner:  NLM     Status:  MEDLINE    
A well-regulated angiogenesis is crucial for proper embryo implantation, embryogenesis, and pregnancy development. Monitoring the presence and distribution of angiogenic regulators in the female reproductive tract and in the early embryo is important for a broader understanding of the molecular aspects of fertility, embryogenesis, and pregnancy. Histidine-rich glycoprotein (HRG) is a glycoprotein involved in angiogenesis. Its presence in the female reproductive tract or in embryos has not previously been studied. Follicular fluid, culture medium, and embryos were obtained from patients undergoing in vitro fertilization (IVF). Biopsies from inner genitalia and placenta were collected at surgery. Histidine-rich glycoprotein presence was investigated by immunohistochemistry and Western blot. Polymerase chain reaction (PCR) was used to determine HRG expression in tissues or by embryos. We identified HRG in follicular fluid, the female reproductive tract, and placenta, as well as in the embryos. Moreover, HRG expression was observed in blastocysts. Thus, the angiogenic properties of HRG might affect fertility.
Sarah Nordqvist; Karin Kårehed; Fredwell Hambiliki; Kjell Wånggren; Anneli Stavreus-Evers; Helena Akerud
Related Documents :
3396704 - Freezing of mammalian embryos without the aid of a programmable freezer.
15358464 - A randomized control comparison study of culture media (htf versus p1) for human in vit...
16725984 - Pregnancy rates with bisected bovine embryos.
3067574 - Interference with gastrulation during the third week of pregnancy as a cause of some fa...
12620994 - Hif1alpha is a critical regulator of secretory differentiation and activation, but not ...
20026294 - Anandamide levels in human female reproductive tissues: solid-phase extraction and meas...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-16
Journal Detail:
Title:  Reproductive sciences (Thousand Oaks, Calif.)     Volume:  17     ISSN:  1933-7205     ISO Abbreviation:  Reprod Sci     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-21     Completed Date:  2010-12-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101291249     Medline TA:  Reprod Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  941-7     Citation Subset:  IM    
Department of Women's and Children's Health, Uppsala University, Uppsala, Sweden.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blastocyst / metabolism*
Embryonic Development / physiology*
Follicular Fluid / metabolism
Genitalia, Female / metabolism*
Placenta / metabolism*
Proteins / genetics,  metabolism*
RNA / chemistry,  genetics
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Proteins; 0/histidine-rich proteins; 63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The Role of Noncoding "Junk DNA" in Cardiovascular Disease.
Next Document:  Pulse pressure and arterial compliance prior to pregnancy and the development of complicated hyperte...