Document Detail


The presence of histidine-rich glycoprotein in the female reproductive tract and in embryos.
MedLine Citation:
PMID:  20639474     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A well-regulated angiogenesis is crucial for proper embryo implantation, embryogenesis, and pregnancy development. Monitoring the presence and distribution of angiogenic regulators in the female reproductive tract and in the early embryo is important for a broader understanding of the molecular aspects of fertility, embryogenesis, and pregnancy. Histidine-rich glycoprotein (HRG) is a glycoprotein involved in angiogenesis. Its presence in the female reproductive tract or in embryos has not previously been studied. Follicular fluid, culture medium, and embryos were obtained from patients undergoing in vitro fertilization (IVF). Biopsies from inner genitalia and placenta were collected at surgery. Histidine-rich glycoprotein presence was investigated by immunohistochemistry and Western blot. Polymerase chain reaction (PCR) was used to determine HRG expression in tissues or by embryos. We identified HRG in follicular fluid, the female reproductive tract, and placenta, as well as in the embryos. Moreover, HRG expression was observed in blastocysts. Thus, the angiogenic properties of HRG might affect fertility.
Authors:
Sarah Nordqvist; Karin Kårehed; Fredwell Hambiliki; Kjell Wånggren; Anneli Stavreus-Evers; Helena Akerud
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-16
Journal Detail:
Title:  Reproductive sciences (Thousand Oaks, Calif.)     Volume:  17     ISSN:  1933-7205     ISO Abbreviation:  Reprod Sci     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-21     Completed Date:  2010-12-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101291249     Medline TA:  Reprod Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  941-7     Citation Subset:  IM    
Affiliation:
Department of Women's and Children's Health, Uppsala University, Uppsala, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Biopsy
Blastocyst / metabolism*
Embryonic Development / physiology*
Female
Follicular Fluid / metabolism
Genitalia, Female / metabolism*
Humans
Immunohistochemistry
Placenta / metabolism*
Pregnancy
Proteins / genetics,  metabolism*
RNA / chemistry,  genetics
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Proteins; 0/histidine-rich proteins; 63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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