| Presence of Apolipoprotein C-III Attenuates Apolipoprotein E-Mediated Cellular Uptake of Cholesterol-Containing Lipid Particles by HepG2 Cells. | |
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MedLine Citation:
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PMID: 21080234 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Apolipoprotein C-III (apoC-III) decreases the apolipoprotein E (apoE)-mediated uptake of lipoprotein remnants by the liver, and a high plasma concentration of apoC-III in VLDL is associated with hypertriglyceridemia and the risk of coronary heart disease. In this study, we prepared lipid emulsions containing triolein, phosphatidylcholine and cholesterol as model particles of lipoproteins, and examined the roles of apoC-III in apoE-mediated uptake of emulsions by HepG2 cells. Cholesterol in emulsion particles enhanced the apoE-mediated uptake via heparan sulfate proteoglycan and LDL receptor-related protein pathways. The amount of apoE bound to emulsion particles was increased by the presence of cholesterol at the particle surface, whereas cholesterol had no effect on the binding amount of apoC-III. Surface cholesterol alleviated the inhibitory effect of apoC-III on apoE incorporation into the emulsion surface. However, ApoC-III almost completely inhibited the apoE-mediated uptake of cholesterol-containing emulsions despite sufficient binding of apoE to emulsions. These findings suggest that apoC-III attenuates the binding of apoE to the lipoprotein surface and apoE-mediated cellular uptake of lipoprotein remnants. Furthermore, cholesterol may affect these functions of apoC-III and apoE involved in the clearance of lipoprotein remnants. |
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Authors:
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Shin-Ya Morita; Atsushi Sakurai; Minoru Nakano; Shuji Kitagawa; Tetsurou Handa |
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Publication Detail:
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Type: Journal Article Date: 2010-11-16 |
Journal Detail:
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Title: Lipids Volume: 46 ISSN: 1558-9307 ISO Abbreviation: Lipids Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: Germany |
Other Details:
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Languages: eng Pagination: 323-32 Citation Subset: IM |
Affiliation:
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Kobe Pharmaceutical University, Higashinada-ku, Kobe, 658-8558, Japan, smorita@kobepharma-u.ac.jp. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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