Document Detail

Preproinsulin I and II mRNA expression in adult rat submandibular glands.
MedLine Citation:
PMID:  10946763     Owner:  NLM     Status:  MEDLINE    
Mammalian salivary glands are known to produce a number of biologically active peptides. The aim of this study was to extend our previous results showing the presence of a biologically active insulin-like immunoreactive peptide in rat salivary glands. In rodents, where two nonallelic and functional insulin genes are expressed, the co-expression of both genes seems to be limited to beta-cells of pancreatic islets or to embryologic developmental processes. We have investigated the expression of insulin genes in rat submandibular glands and in a murine immortalized submandibular cell line, SCA-9. For this purpose, total RNAs were isolated and submitted to reverse transcription. The cDNAs obtained were amplified by a nested polymerase chain reaction using rat preproinsulin I and II primers. Our data show that both preproinsulin I and II mRNAs are expressed in adult rat submandibular glands as well as in the SCA-9 cell line. The identification of salivary gland rat preproinsulin I and II was confirmed by direct sequencing. These results provide, for the first time, evidence for the expression of both preproinsulin I and II mRNA in an extra-pancreatic tissue from adult rodents.
J C Egéa; C Hirtz; R Gross; A D Lajoix; E Traskawka; G Ribes; D D de Périère
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of oral sciences     Volume:  108     ISSN:  0909-8836     ISO Abbreviation:  Eur. J. Oral Sci.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-12-06     Completed Date:  2000-12-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9504563     Medline TA:  Eur J Oral Sci     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  292-6     Citation Subset:  D; IM    
Laboratoire de Physiologie, Faculté d'Odontologie, Montpellier, France.
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MeSH Terms
Base Sequence
Cell Line
Gene Expression
Molecular Sequence Data
Polymerase Chain Reaction
Proinsulin / biosynthesis*,  genetics
Protein Precursors / biosynthesis*,  genetics
RNA, Messenger / analysis
Rats, Wistar
Sequence Analysis, DNA
Submandibular Gland / metabolism*
Reg. No./Substance:
0/Protein Precursors; 0/RNA, Messenger; 11061-68-0/Insulin; 61116-24-3/preproinsulin; 9035-68-1/Proinsulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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