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Prepared the monoclonal antibody associated with hepatocellular carcinoma and analyzed its epitope.
MedLine Citation:
PMID:  21722534     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIM: To prepare the monoclonal antibodies associated with hepatocellular carcinoma (HCC) for diagnosis. METHODS: 3 BALB/c mice were immunized with high metastasis characteristic HCC cells (HCCLM-6). The splenocytes from the immunized mice were fused with murine myeloma cells (Sp2/0). The hybridoma cells were screened by ELISA after coating with the total protein and the culture supernatant of HCCLM-6 cells. The mAbs were characterized by immunohistochemical staining in the HCC tissues, and by indirect immunofIuorescent staining in different cell lines. The antigen and epitope recognized by the mAbs were identified by the screening premade Uni-ZAP human liver cDNA expression library. RESULTS: Twenty-eight hybridoma cells secreting mAbs were established. One clone of the hybridomas, QGA062, secreted specific mAb associated with HCC. The antigen recognized by the mAb QGA062 was identified as fibronectin (FN), and the epitope was localized among the peptide YTVSLVAIKGNQESPK. CONCLUSION: The mAb against a HCC-associated epitope in FN is established and characterized, will be a very useful reagent for diagnosis of HCC.
Authors:
Bing-Fen Yang; Rong Liu; Ying Liu; Jin-Ju Yang; Xiao-Lan Liu; Jian-En Gao; Qi-Hong Sun
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology     Volume:  27     ISSN:  1007-8738     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139110     Medline TA:  Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi     Country:  -    
Other Details:
Languages:  CHI     Pagination:  780-783     Citation Subset:  -    
Affiliation:
Division of Research, Institute of Tuberculosis, No.309 Hospital of PLA, Beijing 100091, China.
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