Document Detail

Preparative separation of pyrogens from proteins by isoelectric focusing using a multicompartment electrolyser with Immobiline membranes.
MedLine Citation:
PMID:  2079048     Owner:  NLM     Status:  MEDLINE    
Due to the nature of lipopolysaccharide endotoxin structures of bacterial pyrogens, their removal from solutions containing therapeutic proteins is often a problem in the pharmaceutical industry. In this report we describe the application of electromotive force to dislodge lipopolysaccharide endotoxins from proteins. This was performed by employing a multicompartment electrolyzer fitted with Immobiline membranes of specified pIs. A thousand-fold reduction of endotoxin could be achieved in the model test system described. This contribution describes the use of a new recycling isoelectric focusing approach without the use of carrier ampholytes.
J Lucas; M Faupel; C Goecking
Related Documents :
10480258 - Separation of proteins and peptides by capillary electrophoresis in acid buffers contai...
23674918 - Enamel matrix protein derivatives: role in periodontal regeneration.
18942678 - Hydroxyethylcellulose-graft-poly (n,n-dimethylacrylamide) copolymer as a multifunctiona...
24947908 - Role of salivary and candidal proteins in denture stomatitis: an exploratory proteomic ...
23999308 - Structure of the red fluorescent protein from a lancelet (branchiostoma lanceolatum): a...
16776338 - Sequential and spatial profiles of apoptosis in ischemic penumbra after two-vein occlus...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Electrophoresis     Volume:  11     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1991-04-30     Completed Date:  1991-04-30     Revised Date:  2006-04-17    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  981-2     Citation Subset:  IM    
Ciba-Geigy Biotechnology Service, Basel.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Isoelectric Focusing*
Isoelectric Point
Membranes, Artificial
Models, Biological
Pyrogens / isolation & purification*
Reg. No./Substance:
0/Membranes, Artificial; 0/Pyrogens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Separation of rat pituitary secretory granules by continuous flow electrophoresis.
Next Document:  Effects of methanol and urea on optical resolution of phenylthiohydantoin-DL-amino acids by micellar...