Document Detail


Preparative separation of pyrogens from proteins by isoelectric focusing using a multicompartment electrolyser with Immobiline membranes.
MedLine Citation:
PMID:  2079048     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Due to the nature of lipopolysaccharide endotoxin structures of bacterial pyrogens, their removal from solutions containing therapeutic proteins is often a problem in the pharmaceutical industry. In this report we describe the application of electromotive force to dislodge lipopolysaccharide endotoxins from proteins. This was performed by employing a multicompartment electrolyzer fitted with Immobiline membranes of specified pIs. A thousand-fold reduction of endotoxin could be achieved in the model test system described. This contribution describes the use of a new recycling isoelectric focusing approach without the use of carrier ampholytes.
Authors:
J Lucas; M Faupel; C Goecking
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Electrophoresis     Volume:  11     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1991-04-30     Completed Date:  1991-04-30     Revised Date:  2006-04-17    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  981-2     Citation Subset:  IM    
Affiliation:
Ciba-Geigy Biotechnology Service, Basel.
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MeSH Terms
Descriptor/Qualifier:
Isoelectric Focusing*
Isoelectric Point
Membranes, Artificial
Models, Biological
Pyrogens / isolation & purification*
Chemical
Reg. No./Substance:
0/Membranes, Artificial; 0/Pyrogens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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