Document Detail


Preparative isolation of terpene trilactones from Ginkgo biloba leaves.
MedLine Citation:
PMID:  16188567     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study investigated and compared some techniques for the preparative isolation of terpene trilactones, including ginkgolides (GA and GB, etc.) and bilobalide (BB), from Ginkgo biloba leaves. The crude Ginkgo biloba L. extracts (GBE) were prepared using an extractor with solvent refluxing operated under an optimal extraction condition. The extraction yield was 20-23% and the purity of terpene trilactones was about 1.0-1.4 wt%. Before the isolation operations, the extracts were dissolved in de-ionized water. The isolation procedures included the method of liquid-liquid extraction and the method of column chromatography. For the method of liquid-liquid extraction using ethyl acetate as the organic solvent operated under the optimal extraction conditions, the purity, concentration ratio, and yield of terpene trilactones were 13.5-18.0%, 15-16, and >99%. For the method of column chromatography, XAD-7HP, XAD-4, and C-18 adsorbents with different polarities were used as the packing materials. Only for the XAD-7HP column, a part of more polar impurities was efficiently separated with the majority of terpene trilactones by a proper step-gradient elution, which resulted in an efficient isolation: the purity, concentration ratio, and yield of terpene trilactones were approximately 20, approximately 15, and approximately 80%. In comparison, the XAD-7HP column achieved the highest purity, but at the expense of the yield of terpene trilactones; on the contrary, the liquid-liquid extraction method, achieving the highest yield but with a slightly lower purity, was proved to be superior to the method of column chromatography in the current isolation stage.
Authors:
Shih-Ming Lai; I-Wen Chen; Ming-Jyi Tsai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1092     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-28     Completed Date:  2006-01-23     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  125-34     Citation Subset:  IM    
Affiliation:
Department of Chemical Engineering, National Yunlin University of Science and Technology, Touliu, Yunlin 640, Taiwan. laism@yuntech.edu.tw
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Chromatography, High Pressure Liquid / methods
Ginkgo biloba / chemistry*
Lactones / isolation & purification*
Plant Leaves / chemistry*
Terpenes / isolation & purification*
Chemical
Reg. No./Substance:
0/Lactones; 0/Terpenes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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