Document Detail

Preparative isolation and purification of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatography.
MedLine Citation:
PMID:  11833643     Owner:  NLM     Status:  MEDLINE    
High-speed counter-current chromatography was applied to the isolation and purification of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza Bunge. The crude salvianolic acid B was obtained by extraction with ethanol-water from S. miltiorrhiza Bunge. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (3:7:1:9, v/v) was successfully performed yielding 342 mg salvianolic acid B at 98% purity from 500 mg of the crude extract in a one-step separation.
Hua-Bin Li; Jia-Ping Lai; Yue Jiang; Feng Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  943     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-02-08     Completed Date:  2002-07-24     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  235-9     Citation Subset:  IM    
Department of Botany, The University of Hong Kong, China.
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MeSH Terms
Benzofurans / analysis,  isolation & purification*
Chromatography, High Pressure Liquid
Countercurrent Distribution / methods*
Salvia / chemistry*
Reg. No./Substance:
0/Benzofurans; 115939-25-8/salvianolic acid B

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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