Document Detail


Preparative and analytical applications of immobilized haemoglobin.
MedLine Citation:
PMID:  7757245     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
New applications of haemoglobin immobilized on Sepharose 4B are proposed for the removal of cell-free, potentially toxic haemoglobin from either natural biological specimens or artificial haemoglobin-containing systems (e.g., blood substitutes, haemosomes) to be employed for biomedical purposes. In a model study, an affinity column of immobilized haemoglobin was used to remove free haemoglobin from blood serum in which controlled haemolysis had been induced. The affinity column retains all the free haemoglobin, does not retain the haemoglobin-haptoglobin complex(es) and leaves the composition of the serum samples unaltered. When immobilized met-haemoglobin is used, haem is transferred readily to albumin, with which it forms a complex. This observation on the one hand shows that immobilized oxyhaemoglobin should be preferred for preparative purposes, and on the other opens the way to the characterization of the haem transfer reaction to albumin by means of immobilized met-haemoglobin. This reaction is difficult to study in solution owing to the overlap of the met-haemoglobin and methaemalbumin spectra.
Authors:
E Chiancone; M Gattoni; C Fronticelli; A Termine; L Silvestri; P Sarti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical applications     Volume:  664     ISSN:  1572-6495     ISO Abbreviation:  J. Chromatogr. B, Biomed. Appl.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-06-26     Completed Date:  1995-06-26     Revised Date:  2007-10-16    
Medline Journal Info:
Nlm Unique ID:  9421796     Medline TA:  J Chromatogr B Biomed Appl     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  89-95     Citation Subset:  IM    
Affiliation:
CNR Centre of Molecular Biology, Department of Biochemical Sciences, University La Sapienza, Rome, Italy.
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MeSH Terms
Descriptor/Qualifier:
Cell-Free System
Chromatography, Affinity / methods*
Hemoglobins / chemistry*,  isolation & purification
Hemolysis
Humans
Chemical
Reg. No./Substance:
0/Hemoglobins

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