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Preparative HPLC separation of underivatized amino acids for isotopic analysis.
MedLine Citation:
PMID:  22125157     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Single-compound analysis of stable or radio-isotopes has found application in a number of fields ranging from archaeology to forensics. Often, the most difficult part of these analyses is the development of a method for isolating the compounds of interest.Here, we describe three complementary preparative HPLC procedures suitable for separating and isolating single amino acids from bone collagen or hair keratin with minimal isotopic contamination. Using preparative reversed-phase, ion-pair, or mixed-mode chromatography of underivatized amino acids in aqueous mobile phases, single amino acids can be isolated and further analyzed using mass spectrometric techniques.
Authors:
Jennifer A Tripp; James S O McCullagh
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  828     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2011-11-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  339-50     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, CA, USA, tripp@sfsu.edu.
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