| Preparative HPLC separation of underivatized amino acids for isotopic analysis. | |
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MedLine Citation:
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PMID: 22125157 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Single-compound analysis of stable or radio-isotopes has found application in a number of fields ranging from archaeology to forensics. Often, the most difficult part of these analyses is the development of a method for isolating the compounds of interest.Here, we describe three complementary preparative HPLC procedures suitable for separating and isolating single amino acids from bone collagen or hair keratin with minimal isotopic contamination. Using preparative reversed-phase, ion-pair, or mixed-mode chromatography of underivatized amino acids in aqueous mobile phases, single amino acids can be isolated and further analyzed using mass spectrometric techniques. |
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Authors:
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Jennifer A Tripp; James S O McCullagh |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 828 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
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Created Date: 2011-11-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 339-50 Citation Subset: IM |
Affiliation:
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Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, CA, USA, tripp@sfsu.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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