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Preparation of unnatural amino acids with ammonia-lyases and 2,3-aminomutases.
MedLine Citation:
PMID:  21956553     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Ammonia-lyases catalyze a wide range of processes leading to α,β-unsaturated compounds by elimination of ammonia. In this chapter, ammonia-lyases are reviewed with major emphasis on their synthetic applications in stereoselective preparation of unnatural amino acids. Besides the synthesis of various unnatural α-amino acids with the aid of phenylalanine ammonia-lyases (PALs) utilizing the 3,5-dihydro-5-methylidene-4H-imidazol-4-one (MIO) prosthetic groups, the biotransformations leading to various unnatural β-amino acids with phenylalanine 2,3-aminomutases using the same catalytic MIO prosthetic group are discussed. Cloning, production, purification, and biotransformation protocols for PAL are described in detail.
Authors:
László Poppe; Csaba Paizs; Klaudia Kovács; Florin-Dan Irimie; Beáta Vértessy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  794     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2011-09-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3-19     Citation Subset:  IM    
Affiliation:
Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, Budapest, Hungary, poppe@mail.bme.hu.
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