Document Detail


Preparation of tetramethylrhodaminyl-phalloidin and uptake of the toxin into short-term cultured hepatocytes by endocytosis.
MedLine Citation:
PMID:  6840214     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A fluorescent phallotoxin with high photostability, tetramethylrhodaminyl-phalloidin (Rh-phalloidin), has been prepared. The affinity of this compound to rabbit muscle actin has been determined to be about 6 times lower than that of phalloidin. In freshly isolated hepatocytes the internalized fluorescent toxin stains the cellular actin. In contrary, there is no actin staining visible in cultured hepatocytes. Short-term cultured hepatocytes (5 h of culturing) incorporate the toxin by endocytosis; it is kept sealed in the endocytotic vesicles, which are usually found accumulated at the sites where cells touch after reaggregation.
Authors:
H Faulstich; H Trischmann; D Mayer
Related Documents :
15174094 - Chimeric molecule il-6/soluble il-6 receptor is a potent mitogen for fetal hepatocytes.
1718514 - Effect of culture duration on hepatocyte subcellular membranes involved in endocytosis.
21951284 - Spontaneous and induced osteoclastogenic behaviour of human peripheral blood mononuclea...
21731714 - Short-term serum-free culture reveals that inhibition of gsk3β induces the tumor-like g...
10885714 - Semiquantitative culture of subcutaneous segment for conservative diagnosis of intravas...
11500844 - Short- and long-chain natural polyamines play specific roles in adult cricket neuroblas...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental cell research     Volume:  144     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1983 Mar 
Date Detail:
Created Date:  1983-06-10     Completed Date:  1983-06-10     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  73-82     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Cells, Cultured
Endocytosis*
Liver / cytology*,  metabolism
Male
Oligopeptides / metabolism*
Phalloidine / metabolism*,  pharmacology
Rats
Rats, Inbred Strains
Rhodamines
Chemical
Reg. No./Substance:
0/Actins; 0/Oligopeptides; 0/Rhodamines; 17466-45-4/Phalloidine; 62669-72-1/tetramethylrhodamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Isolation and preliminary characterization of the synaptonemal complex from rat pachytene spermatocy...
Next Document:  Isolation and characterization of Chinese hamster cells defective in cell-cell coupling via gap junc...