| Preparation of raf-oncogene-specific antiserum with raf protein produced in E. coli. | |
| | |
MedLine Citation:
|
PMID: 3277669 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In this report we describe the expression of v-raf protein in E. coli using a tryptophan-promoter-driven expression vector and its immunological characterization by anti-peptide sera. The purified recombinant protein was used to produce raf-specific antibodies which are suitable for studying v-raf and c-raf proteins in vitro and in vivo in a variety of species ranging from mouse to man. |
| | |
Authors:
|
W Kolch; A M Schultz; H Oppermann; U R Rapp |
Related Documents
:
|
6158089 - Interaction of human polyclonal ige and igg from different species with protein a from ... 22806459 - Optimization of protein sample preparation for two-dimensional electrophoresis. 2086449 - Polyanions inhibit binding of human autoantibodies to certain cellular proteins. 22273739 - Structural and dynamic aspects of protein clocks: how can they be so slow and stable? 22575289 - A synthetic protein g adsorbent based on the multi-component ugi reaction for the purif... 10095789 - Detection of membrane-bound cytokinin-binding proteins in arabidopsis thaliana cells. 7853889 - Heat shock protein (hsp 72) expression in patients undergoing cardiac operations. 11171359 - A comparative analysis of the evolutionary patterning and mechanistic bases of lactate ... 6180589 - Histochemical basis of the vertebrate epidermis differentiation in the comparative aspe... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 949 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1988 Feb |
Date Detail:
|
Created Date: 1988-04-01 Completed Date: 1988-04-01 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 233-9 Citation Subset: IM |
Affiliation:
|
Laboratory of Viral Carcinogenesis, NCI-Frederick Cancer Research Facility, MD 21701. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Antibody Specificity Chemical Precipitation Cloning, Molecular Escherichia coli / genetics Molecular Weight Oncogenes* Peptide Fragments / immunology Recombinant Proteins / immunology* Structure-Activity Relationship |
| Grant Support | |
ID/Acronym/Agency:
|
N01-C0-74101//PHS HHS |
| Chemical | |
Reg. No./Substance:
|
0/Peptide Fragments; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Gene response upon illumination in forming mRNA encoding peroxisomal glycollate oxidase.
Next Document: Differently bound copper(I) in yeast Cu8-thionein.