Document Detail


Preparation of radioactively labeled synthetic sn-1,2-diacylglycerols for studies of lipid metabolism.
MedLine Citation:
PMID:  7710117     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method was developed for the synthesis of stereo-specific sn-1,2-diacylglycerols radioactively labeled on the glycerol backbone. This method includes the acylation of L-[U-14C]glycerol 3-phosphate with fatty acid anhydrides in the presence of dimethylaminopyridine and the tetraethylammonium salt of the fatty acid used for the acylation. The phosphatidic acid obtained from this synthesis was converted to sn-1,2-diacyl[U-14C]glycerol by the action of phospholipase C. When di10:0-, di-18:1-, di-18:2-, and di-22:1-diacyl[U-14C]glycerols made by this method were provided as substrates to microsome preparations from developing cotyledons of safflower (Carthamus tinctorius), they were all incorporated into phosphatidylcholine by the action of cholinephosphotransferase. The time course of the incorporation into phosphatidylcholine showed no differences for these four substrates and the initial rates were comparable.
Authors:
G Vogel; J Browse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  224     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-05-10     Completed Date:  1995-05-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  61-7     Citation Subset:  IM    
Affiliation:
Institute of Biological Chemistry, Washington State University, Pullman 99164-6340.
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MeSH Terms
Descriptor/Qualifier:
Carbon Radioisotopes / diagnostic use
Diglycerides / chemical synthesis*
Isotope Labeling*
Lipid Metabolism*
Phosphatidylcholines / metabolism
Chemical
Reg. No./Substance:
0/1,2-diacylglycerol; 0/Carbon Radioisotopes; 0/Diglycerides; 0/Phosphatidylcholines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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