Document Detail

Preparation and properties of fluorescent glycosaminoglycuronans labeled with 5-aminofluorescein.
MedLine Citation:
PMID:  7116357     Owner:  NLM     Status:  MEDLINE    
The uronic acid residues of all known glycosaminoglycuronans reacted with 5-aminofluorescein to yield fluorescent glycosaminoglycuronan derivatives, which showed fluorescence characteristics identical to those of fluorescein or 5-acetamidofluorescein. The fluorescent products could be purified by chromatography on Octyl-Sepharose; three preparations of labeled chondroitin 6-sulfate having different degrees of substitution, and a labeled heparin were obtained. Fluorescent hyaluronic acid containing labeled and unlabeled molecules was digested with testicular hyaluronidase to give fluorescent oligosaccharides. Fluorescent chondroitin 6-sulfate was treated with chondroitinase AC to give a nonfluorescent disaccharide and minor proportion of fluorescent octasaccharide. Fluorescent heparin retained its anticoagulant activity, which was similar to that of the starting heparin; its half-life in circulating rabbit blood was 36 min (by fluorometry) and 45 min (by clotting-time assay).
A Ogamo; K Matsuzaki; H Uchiyama; K Nagasawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Carbohydrate research     Volume:  105     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  1982 Jul 
Date Detail:
Created Date:  1982-12-02     Completed Date:  1982-12-02     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  69-85     Citation Subset:  IM    
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MeSH Terms
Chondroitinases and Chondroitin Lyases / metabolism
Glycosaminoglycans* / blood,  chemical synthesis
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Reg. No./Substance:
0/Fluoresceins; 0/Glycosaminoglycans; 3326-34-9/5-aminofluorescein; EC 4.2.2.-/Chondroitinases and Chondroitin Lyases

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