Document Detail

Preparation of optically pure tert-leucine by penicillin G acylase-catalyzed resolution.
MedLine Citation:
PMID:  16707334     Owner:  NLM     Status:  MEDLINE    
Penicillin G acylase, from Kluyvera citrophila, was used in kinetic resolution of DL-tert-leucine. N-phenylacetylated-DL-tert-leucine, chemically synthesized from DL-tert-leucine, was enantioselctively hydrolyzed by penicillin G acylase to obtain L-tert-leucine, D-tert-leucine was prepared by acid-catalyzed hydrolysis of the remaining substrate. The total yields of D-tert-leucine and L-tert-leucine are 80.6% and 83.1%, respectively. The enantiomeric excess of the two products, D-tert-leucine and L-tert-leucine, are 98.5% and 99%. This is a practical way for the preparation of D-tert-leucine and L-tert-leucine.
Shu-Lai Liu; Qing-Xun Song; Dong-Zhi Wei; Ye-Wang Zhang; Xue-Dong Wang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Preparative biochemistry & biotechnology     Volume:  36     ISSN:  1082-6068     ISO Abbreviation:  Prep. Biochem. Biotechnol.     Publication Date:  2006  
Date Detail:
Created Date:  2006-05-18     Completed Date:  2007-01-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9607037     Medline TA:  Prep Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  235-41     Citation Subset:  IM    
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, P. R. China.
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MeSH Terms
Hydrogen-Ion Concentration
Kluyvera / enzymology
Leucine / analogs & derivatives*,  chemical synthesis*,  chemistry
Molecular Structure
Optical Rotation
Penicillin Amidase / metabolism*
Penicillin G / metabolism
Reg. No./Substance:
61-33-6/Penicillin G; 61-90-5/Leucine; EC Amidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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