| Preparation of optically pure tert-leucine by penicillin G acylase-catalyzed resolution. | |
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MedLine Citation:
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PMID: 16707334 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Penicillin G acylase, from Kluyvera citrophila, was used in kinetic resolution of DL-tert-leucine. N-phenylacetylated-DL-tert-leucine, chemically synthesized from DL-tert-leucine, was enantioselctively hydrolyzed by penicillin G acylase to obtain L-tert-leucine, D-tert-leucine was prepared by acid-catalyzed hydrolysis of the remaining substrate. The total yields of D-tert-leucine and L-tert-leucine are 80.6% and 83.1%, respectively. The enantiomeric excess of the two products, D-tert-leucine and L-tert-leucine, are 98.5% and 99%. This is a practical way for the preparation of D-tert-leucine and L-tert-leucine. |
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Authors:
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Shu-Lai Liu; Qing-Xun Song; Dong-Zhi Wei; Ye-Wang Zhang; Xue-Dong Wang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Preparative biochemistry & biotechnology Volume: 36 ISSN: 1082-6068 ISO Abbreviation: Prep. Biochem. Biotechnol. Publication Date: 2006 |
Date Detail:
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Created Date: 2006-05-18 Completed Date: 2007-01-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9607037 Medline TA: Prep Biochem Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 235-41 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, P. R. China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Catalysis Hydrogen-Ion Concentration Hydrolysis Kluyvera / enzymology Leucine / analogs & derivatives*, chemical synthesis*, chemistry Molecular Structure Optical Rotation Penicillin Amidase / metabolism* Penicillin G / metabolism |
| Chemical | |
Reg. No./Substance:
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61-33-6/Penicillin G; 61-90-5/Leucine; EC 3.5.1.11/Penicillin Amidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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