Document Detail


Preparation of new omega-aldehydoalkyl 1-thio-D-glycopyranosides, and their coupling to bovine serum albumin by reductive alkylation.
MedLine Citation:
PMID:  7060055     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Synthesis of two omega-aldehydoalkyl 1-thioglycosides of D-glucopyranose and of D-galactopyranose is described. 3-Oxopropyl and 2-oxoethyl 1-thioglycosides were prepared by treating a tetra-O-acetyl-1-thioaldose with either acrolein or 2-bromo-acetaldehyde, followed by O-deacetylation under mild conditions. These omega-aldehydoalkyl 1-thioglycosides were successfully attached to bovine serum albumin (BSA) by reductive alkylation as described previously. With the 3-oxopropyl 1-thioglycosides, much higher levels of sugar attachment (e.g., approximately 80 mol of sugar per mol of BSA) were attained than hitherto possible with any sugar derivative tested.
Authors:
R T Lee; Y C Lee
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Carbohydrate research     Volume:  101     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  1982 Feb 
Date Detail:
Created Date:  1982-05-21     Completed Date:  1982-05-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  49-55     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alkylation
Animals
Cattle
Indicators and Reagents
Kinetics
Magnetic Resonance Spectroscopy
Protein Binding
Serum Albumin, Bovine*
Thioglycosides / chemical synthesis*
Grant Support
ID/Acronym/Agency:
AM9970/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 0/Serum Albumin, Bovine; 0/Thioglycosides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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