Document Detail


Preparation of mouse feeder cells for fusion and cloning.
MedLine Citation:
PMID:  18265078     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To maximize the yield of hybrids from fusion and cloning procedures, feeder cells are required to be cocultured with the hybrids, while hybrid cell density is low. Mouse peritoneal cells, most of which are macrophages, have been found to be convenient and effective feeder cells which are a source of soluble growth factors for hybridoma cells.
Authors:
S A Fuller; M Takahashi; J G Hurrell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]     Volume:  Chapter 11     ISSN:  1934-3647     ISO Abbreviation:  Curr Protoc Mol Biol     Publication Date:  2001 May 
Date Detail:
Created Date:  2008-02-11     Completed Date:  2008-02-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8908160     Medline TA:  Curr Protoc Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit11.6     Citation Subset:  IM    
Affiliation:
Allelix Inc., Mississauga, Ontario.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / biosynthesis*,  isolation & purification*
Coculture Techniques*
Hybridomas / immunology*
Mice
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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