Document Detail

Preparation of mono- and diacylglycerols by enzymatic esterification of glycerol with conjugated linoleic acid in hexane.
MedLine Citation:
PMID:  15834163     Owner:  NLM     Status:  MEDLINE    
Esterification of glycerol with conjugated linoleic acid (CLA) was carried out in hexane. Lipase from Rhizomucor miehei provided a high degree of esterification (80%) in 8 h at 50 degrees C when used at 15% (w/w) in a system containing a 1:2 molar ratio of glycerol to free fatty acids. Esterification levels >80% were obtained in 8 h at 40 degrees C with 15% (w/w) lipase from Candida antarctica at the same molar ratio of reactants. The extent of esterification of CLA was >90% after 4 h of reaction at 50 degrees C with a 5% (w/w) loading of either R. miehei or C. antarctica lipase, together with a 1:1 molar ratio of substrates. Both enzymes incorporated the original CLA as acylglycerol residues in primarily 1,3-diacylglycerol and 1-monoacylglycerol. The CLA-rich acylglycerols can be employed as emulsifiers or as substitutes for natural fats and oils.
C E Martinez; J C Vinay; R Brieva; C G Hill; H S Garcia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  125     ISSN:  0273-2289     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-18     Completed Date:  2005-07-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  63-75     Citation Subset:  IM    
Coordinación de Posgrado e Investigación, Instituto Tecnológico de Tuxtepec, Tuxtepec, Oax. 68300, Calz. Dr. Víctor Bravo Ahuja s/n, Mexico.
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MeSH Terms
Chromatography, High Pressure Liquid
Diglycerides / biosynthesis*
Enzymes, Immobilized
Food Technology / methods
Glycerides / biosynthesis*,  chemistry
Glycerol / metabolism*
Linoleic Acids, Conjugated / metabolism*
Lipase / metabolism*
Rhizomucor / enzymology
Reg. No./Substance:
0/Diglycerides; 0/Enzymes, Immobilized; 0/Glycerides; 0/Hexanes; 0/Linoleic Acids, Conjugated; 56-81-5/Glycerol; EC

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