Document Detail


Preparation of methyl ursodeoxycholate via microbial reduction of methyl 7-ketolithocholate in an anaerobic interface bioreactor.
MedLine Citation:
PMID:  16232975     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
An interface bioreactor, which is a device for the microbial transformation of water-insoluble substrates, was applied to an anaerobic bioconversion for the first time. Methyl 7-ketolithocholate [Me-7KLCA] was reduced with the human intestinal bacterium Eubacterium aerofaciens JCM 7790 in a convenient anaerobic interface bioreactor using a nutrient agar plate placed in a GasPak pouch. The resulting methyl ursodeoxycholate [Me-UDCA] is a precursor of ursodeoxycholic acid, which is used as a cholesterol gallstone-dissolving agent. The reaction conditions were optimized, and ABCM medium and dihexyl ether were selected as the best carrier and reaction solvent, respectively. The toxicity of the bile acid esters toward the human intestinal bacterium was effectively alleviated in the interface bioreactor, in which the maximal concentrations of Me-7KLCA and Me-UDCA in the dihexyl ether layer respectively reached to 12.0 and 6.1 g/l.
Authors:
S Oda; T Sugai; H Ohta
Related Documents :
9008525 - Serum igm monoclonal autoantibody binding to the 301 to 314 amino acid epitope of beta-...
7327385 - Oral ursodeoxycholic acid tolerance test in patients with digestive disease.
10906645 - Effects of glycolic acid on light-induced skin pigmentation in asian and caucasian subj...
6634915 - Studies on flufenamic acid capsules and tablets.
3693125 - The structure of manumycin. iii. absolute configuration and conformational studies.
312105 - Hyperalgesia after treatment of mice with prostaglandins and arachidonic acid and its a...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  91     ISSN:  1389-1723     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2001  
Date Detail:
Created Date:  2005-10-19     Completed Date:  2005-11-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  202-7     Citation Subset:  -    
Affiliation:
Technical Research Laboratory, Kansai Paint Co. Ltd., 4-17-1 Higashi-Yawata, Hiratsuka, Kanagawa 254-8562, Japan. odas@als.kansai.co.jp
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Characterization of lighted upflow anaerobic sludge blanket (LUASB) method under sulfate-rich condit...
Next Document:  Methane fermentation of bean curd refuse.