| Preparation of methyl ursodeoxycholate via microbial reduction of methyl 7-ketolithocholate in an anaerobic interface bioreactor. | |
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MedLine Citation:
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PMID: 16232975 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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An interface bioreactor, which is a device for the microbial transformation of water-insoluble substrates, was applied to an anaerobic bioconversion for the first time. Methyl 7-ketolithocholate [Me-7KLCA] was reduced with the human intestinal bacterium Eubacterium aerofaciens JCM 7790 in a convenient anaerobic interface bioreactor using a nutrient agar plate placed in a GasPak pouch. The resulting methyl ursodeoxycholate [Me-UDCA] is a precursor of ursodeoxycholic acid, which is used as a cholesterol gallstone-dissolving agent. The reaction conditions were optimized, and ABCM medium and dihexyl ether were selected as the best carrier and reaction solvent, respectively. The toxicity of the bile acid esters toward the human intestinal bacterium was effectively alleviated in the interface bioreactor, in which the maximal concentrations of Me-7KLCA and Me-UDCA in the dihexyl ether layer respectively reached to 12.0 and 6.1 g/l. |
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Authors:
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S Oda; T Sugai; H Ohta |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bioscience and bioengineering Volume: 91 ISSN: 1389-1723 ISO Abbreviation: J. Biosci. Bioeng. Publication Date: 2001 |
Date Detail:
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Created Date: 2005-10-19 Completed Date: 2005-11-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888800 Medline TA: J Biosci Bioeng Country: Japan |
Other Details:
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Languages: eng Pagination: 202-7 Citation Subset: - |
Affiliation:
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Technical Research Laboratory, Kansai Paint Co. Ltd., 4-17-1 Higashi-Yawata, Hiratsuka, Kanagawa 254-8562, Japan. odas@als.kansai.co.jp |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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