Document Detail


Preparation of an insulin conjugate with beta-galactosidase from E. coli for immunoenzyme assay
MedLine Citation:
PMID:  3083825     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A modified procedure has been worked out for preparing a conjugate of porcine insulin with E. coli beta-galactosidase employing a heterobifunctional reagent, N-hydroxysuccinimidyl m-maleimidobenzoate. Optimal conditions for insulin acylation and subsequent coupling with beta-galactosidase were selected that afforded the conjugate in a high yield. The ability of the modified antigen to react with antibody was evaluated in the reaction of conjugate binding with immobilized monoclonal antibody to insulin. The conjugate almost completely retained the enzymatic activity and reacted with high specificity with the antibody to insulin. The conjugate can be used in competitive ELISA of insulin.
Authors:
A N Markarian; T V Cherednikova; B B Dzantiev
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Bioorganicheskaia khimiia     Volume:  12     ISSN:  0132-3423     ISO Abbreviation:  Bioorg. Khim.     Publication Date:  1986 Mar 
Date Detail:
Created Date:  1986-04-28     Completed Date:  1986-04-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7804941     Medline TA:  Bioorg Khim     Country:  USSR    
Other Details:
Languages:  rus     Pagination:  327-31     Citation Subset:  IM    
Vernacular Title:
Poluchenie kon''iugata insulina s beta-galaktozidazoĭ iz E. coli dlia tseleĭ immunofermentnogo analiza.
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MeSH Terms
Descriptor/Qualifier:
Chemical Phenomena
Chemistry
Enzyme-Linked Immunosorbent Assay*
Escherichia coli / enzymology
Galactosidases / diagnostic use*
Insulin / analysis*
beta-Galactosidase / diagnostic use*
Chemical
Reg. No./Substance:
11061-68-0/Insulin; 12584-58-6/insulin, neutral; EC 3.2.1.-/Galactosidases; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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