| Preparation of insoluble transmembrane peptides: glycophorin-A, prion (110-137), and FGFR (368-397). | |
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MedLine Citation:
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PMID: 10415099 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A general procedure for the reliable preparation of large quantities of insoluble transmembrane peptides has been developed. Optimal couplings were obtained during synthesis by using high-temperature couplings in conjunction with O-(7-azabenzotriazol-1-yl)-1,1,3, 3-tetramethyluronium hexafluorophosphate (HATU) activation. Improved purification schemes were developed that use reverse- phase HPLC on a C1 column and elution with a 2:1 mixture of 1-propanol:1-butanol. Using these methods three very different transmembrane peptides all longer than 25 amino acids have been prepared: glycophorin-A, prion (110-137), and fibroblast growth factor receptor (368-397). |
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Authors:
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K J Glover; P M Martini; R R Vold; E A Komives |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Analytical biochemistry Volume: 272 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1999 Aug |
Date Detail:
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Created Date: 1999-08-30 Completed Date: 1999-08-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 270-4 Citation Subset: IM |
Copyright Information:
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Copyright 1999 Academic Press. |
Affiliation:
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Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0601, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Chromatography, High Pressure Liquid / methods Glycophorin / chemical synthesis*, chemistry Indicators and Reagents Mass Spectrometry / methods Molecular Sequence Data Peptide Fragments / chemical synthesis*, chemistry Prions / chemical synthesis*, chemistry, isolation & purification Receptors, Fibroblast Growth Factor / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Glycophorin; 0/Indicators and Reagents; 0/Peptide Fragments; 0/Prions; 0/Receptors, Fibroblast Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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