| Preparation of the inguinal fat pad for perfusion in situ in the rat: a surgical technique that preserves continuous blood flow. | |
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MedLine Citation:
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PMID: 11040872 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We developed a surgical technique for preparing the inguinal fat pad of rats for perfusion that preserves continuous blood flow to the tissue. Fatty acid uptake from the fat pads of fed rats (46.1 +/- 2.1% of arterial supply, n = 82) and release from those of fasted (48 h) animals (51.3 +/- 3.1% of supply, n = 69) occurred principally via the free fatty acid component of the blood; levels of triglycerides, cholestryl esters, and phospholipids did not change significantly. Venous blood flow in perfusions using blood from fed rats was 83.3 +/- 1.7 mL/min and 83.1 +/- 1.5 microL/min in experiments involving fasted donors. Values for arterial (A) and venous (V) pH (A, 7.41 +/- 0.03; V, 7.32 +/- 0.04), pO2 (A, 151.6 +/- 15.7 mm Hg; V, 34.7 +/- 9.2 mm Hg), pCO2 (A, 30.8 +/- 6.6 mm Hg; V, 58.2 +/- 5.2 mm Hg), and hematocrit (A, 44.6 +/- 1.2%; V, 45.7 +/- 1.2%) were unchanged throughout the course of the perfusions. Fat pad and blood perfusates were maintained at 37 degrees C. Tissue homogenates revealed that the total fatty acid content of fat pads from fed rats (333.5 +/- 0.3 mg/g tissue) differed significantly from that in fasted animals (260.7 +/- 0.7 mg/g tissue; P < 0.001). Our technique likely will have many uses in the study of lipid transport and metabolism, hyperlipidemia, and cancer-associated cachexia. |
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Authors:
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R T Dauchy; D E Blask; L A Sauer |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Contemporary topics in laboratory animal science / American Association for Laboratory Animal Science Volume: 39 ISSN: 1060-0558 ISO Abbreviation: Contemp Top Lab Anim Sci Publication Date: 2000 Sep |
Date Detail:
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Created Date: 2000-11-13 Completed Date: 2000-11-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9204153 Medline TA: Contemp Top Lab Anim Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 29-33 Citation Subset: IM |
Affiliation:
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Laboratory of Experimental Neuroendocrinology/Oncology, Bassett Research Institute, Cooperstown, NY 13326-1394, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adipose Tissue
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blood supply*,
surgery Animals Blood Donors Cholesterol Esters / blood Fatty Acids / analysis, blood Groin / blood supply, surgery Hematocrit / veterinary Hydrogen-Ion Concentration Male Phospholipids / blood Rats Rats, Inbred BUF / surgery* Rats, Sprague-Dawley Specific Pathogen-Free Organisms Surgery, Veterinary / methods* Triglycerides / blood |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA76197/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cholesterol Esters; 0/Fatty Acids; 0/Phospholipids; 0/Triglycerides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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