| Preparation and in vivo evaluation of apatite/collagen packed composite by alternate immersion method and Newton press. | |
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MedLine Citation:
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PMID: 19165766 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Further development of bio-compatible, bio-absorbable, and osteo-conductive bio-materials is desired for bone grafts in dental and medical clinics. One candidate material might be a high-density apatite/collagen composite, which cures relatively large bone defects. To produce such a composite, we freeze-dried type I collagen solution, cross-linked the formed sponge by 2 wt % glutaraldehyde, immersed the insoluble sponge in CaCl(2) and Na(2)HPO(4) solutions alternately five times, and compacted the sponge by Newton press at 5000 kgf. For comparison, cross-linked collagen without alternate immersion was also pressed. SEM/EPMA, XRD, and FTIR analyses clarified that alternate immersion successfully coated the collagen sponge with hydroxyapatite. Packed apatite/collagen composite and collagen disks 6 mm in diameter and 0.5 mm in height were implanted in the subperiostea of rabbit tibiae for 2, 4, 8, and 12 weeks to assess bio-compatibility, bio-absorbability, and osteo-conductivity. Histological observations showed that the packed apatite/collagen composite was biocompatible, osteo-conductive for up to 8 weeks, and largely bio-absorbed at 12 weeks, while the packed collagen sponge caused an undesirable foreign body reaction, which worsened with the implantation period. The overall findings suggest that this packed apatite/collagen composite might be used as a new bio-absorbable bone graft material. |
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Authors:
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Masayuki Taira; Takashi Nezu; Kaori Sasaki; Setsuo Saitoh; Tadayoshi Kagiya; Hidemitsu Harada; Yukyo Takada; Yoshima Araki |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical materials research. Part B, Applied biomaterials Volume: 90 ISSN: 1552-4981 ISO Abbreviation: J. Biomed. Mater. Res. Part B Appl. Biomater. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-08 Completed Date: 2009-12-16 Revised Date: 2013-04-05 |
Medline Journal Info:
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Nlm Unique ID: 101234238 Medline TA: J Biomed Mater Res B Appl Biomater Country: United States |
Other Details:
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Languages: eng Pagination: 566-73 Citation Subset: IM |
Affiliation:
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Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry, Morioka, Japan. mtaira@iwate-med.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Absorbable Implants Animals Apatites / chemistry Biocompatible Materials / chemistry* Bone Substitutes / chemistry Calcium Phosphates / chemistry* Collagen / chemistry Equipment Design Foreign-Body Reaction Glutaral / chemistry Male Materials Testing Osteoclasts / metabolism Prostheses and Implants Rabbits Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Apatites; 0/Biocompatible Materials; 0/Bone Substitutes; 0/Calcium Phosphates; 111-30-8/Glutaral; 9007-34-5/Collagen; 97Z1WI3NDX/calcium phosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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