Document Detail

Preparation and in vivo evaluation of apatite/collagen packed composite by alternate immersion method and Newton press.
MedLine Citation:
PMID:  19165766     Owner:  NLM     Status:  MEDLINE    
Further development of bio-compatible, bio-absorbable, and osteo-conductive bio-materials is desired for bone grafts in dental and medical clinics. One candidate material might be a high-density apatite/collagen composite, which cures relatively large bone defects. To produce such a composite, we freeze-dried type I collagen solution, cross-linked the formed sponge by 2 wt % glutaraldehyde, immersed the insoluble sponge in CaCl(2) and Na(2)HPO(4) solutions alternately five times, and compacted the sponge by Newton press at 5000 kgf. For comparison, cross-linked collagen without alternate immersion was also pressed. SEM/EPMA, XRD, and FTIR analyses clarified that alternate immersion successfully coated the collagen sponge with hydroxyapatite. Packed apatite/collagen composite and collagen disks 6 mm in diameter and 0.5 mm in height were implanted in the subperiostea of rabbit tibiae for 2, 4, 8, and 12 weeks to assess bio-compatibility, bio-absorbability, and osteo-conductivity. Histological observations showed that the packed apatite/collagen composite was biocompatible, osteo-conductive for up to 8 weeks, and largely bio-absorbed at 12 weeks, while the packed collagen sponge caused an undesirable foreign body reaction, which worsened with the implantation period. The overall findings suggest that this packed apatite/collagen composite might be used as a new bio-absorbable bone graft material.
Masayuki Taira; Takashi Nezu; Kaori Sasaki; Setsuo Saitoh; Tadayoshi Kagiya; Hidemitsu Harada; Yukyo Takada; Yoshima Araki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research. Part B, Applied biomaterials     Volume:  90     ISSN:  1552-4981     ISO Abbreviation:  J. Biomed. Mater. Res. Part B Appl. Biomater.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-08     Completed Date:  2009-12-16     Revised Date:  2013-04-05    
Medline Journal Info:
Nlm Unique ID:  101234238     Medline TA:  J Biomed Mater Res B Appl Biomater     Country:  United States    
Other Details:
Languages:  eng     Pagination:  566-73     Citation Subset:  IM    
Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry, Morioka, Japan.
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MeSH Terms
Absorbable Implants
Apatites / chemistry
Biocompatible Materials / chemistry*
Bone Substitutes / chemistry
Calcium Phosphates / chemistry*
Collagen / chemistry
Equipment Design
Foreign-Body Reaction
Glutaral / chemistry
Materials Testing
Osteoclasts / metabolism
Prostheses and Implants
Time Factors
Reg. No./Substance:
0/Apatites; 0/Biocompatible Materials; 0/Bone Substitutes; 0/Calcium Phosphates; 111-30-8/Glutaral; 9007-34-5/Collagen; 97Z1WI3NDX/calcium phosphate

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