| Preparation of immunomagnetic iron-dextran nanoparticles and application in rapid isolation of E.coli O157:H7 from foods. | |
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MedLine Citation:
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PMID: 15968716 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To prepare a kind of magnetic iron-dextran nanoparticles that was coated with anti-E.coli O157:H7 IgG, analyze its application conditions, and try to use it to isolate E.coli O157:H7 from foods. METHODS: Magnetic iron-dextran nanoparticles were prepared by the reaction of a mixture of ferric and ferrous ions with dextran polymers under alkaline conditions. The particles were coated with antiserum against E.coli O157:H7 by the periodate oxidation-borohydride reduction procedure. The oxidation time, amount of antibody coating the particles, amount of nanoparticles, incubation time and isolation time were varied to determine their effects on recovery of the organisms. Finally, the optimum conditions for isolating E.coli O157:H7 from food samples were established. RESULTS: E.coli O157:H7 can be isolated from samples within 15 min with the sensitivity of 10(1) CFU/mL or even less. In the presence of 10(8) CFU/mL of other organisms, the sensitivity is 10(1)-10(2) CFU/mL. Nonspecific binding of other bacteria to the particles was not observed. Two and a half hours of enrichment is enough for the particles to detect the target from the food samples inoculated with 1 CFU/g. CONCLUSION: Isolation of target bacteria by immunomagnetic nanoparticles is an efficient method with high sensitivity and specificity. The technique is so simple that it can be operated in lab and field even by untrained personnel. |
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Authors:
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Hui-Li Duan; Zhi-Qiang Shen; Xin-Wei Wang; Fu-Huan Chao; Jun-Wen Li |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 11 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2005-06-21 Completed Date: 2005-10-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
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Languages: eng Pagination: 3660-4 Citation Subset: IM |
Affiliation:
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Department of Environmental Health, Institute of Hygiene and Environmental Medicine, Tianjin 300050, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Escherichia coli O157
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isolation & purification* Food Contamination / prevention & control Food Microbiology* Immunomagnetic Separation / methods* Iron-Dextran Complex* Nanostructures* |
| Chemical | |
Reg. No./Substance:
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9004-66-4/Iron-Dextran Complex |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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