Document Detail


Preparation of an immunoaffinity column for the clean-up of fermented food samples contaminated with citrinin.
MedLine Citation:
PMID:  23157620     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
An immunoaffinity column (IAC) for the clean-up of citrinin-contaminated food samples was prepared by using silica gel immobilised with a anti-citrinin antibody. Antibodies produced against citrinin-bovine serum albumin (BSA) were covalently immobilised onto a silica-based solid support to prepare the IAC. The selective extraction and clean-up ability of the IAC was evaluated by capillary zone electrophoresis coupled with ultraviolet detection. Clean-up conditions such as eluting solutions kind, concentrations, pH and volumes were optimised for citrinin by using the IAC. IACs were applied to citrinin-contaminated foods such as red yeast rice and monascus colour. The method's performance was acceptable in terms of recoveries, which ranged from 80.4% to 97.1% for citrinin-spiked samples at levels of 50, 100 and 200 µg kg(-1), and the relative standard deviation ranged from 5.3% to 10.5%.
Authors:
Dan Zhu; Hongyan Zhang; Xin Bing
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-16
Journal Detail:
Title:  Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment     Volume:  -     ISSN:  1944-0057     ISO Abbreviation:  Food Addit Contam Part A Chem Anal Control Expo Risk Assess     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101485040     Medline TA:  Food Addit Contam Part A Chem Anal Control Expo Risk Assess     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
a College of Life Science , Shandong Normal University , Jinan , P. R. China.
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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