| Preparation of heterodifunctional polyethyleneglycols: network formation, characterization, and cell culture analysis. | |
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MedLine Citation:
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PMID: 11556739 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Polyethyleneglycols (PEG) are employed extensively in the development of biomaterials; however, the hydroxyl groups in PEG-diols have very limited chemical activity. We developed a synthesis scheme for a library of heterodifunctional PEG (hPEG) with two distinct terminal moieties to improve the reactivity and physicochemical properties of PEG. hPEG were employed in the formulation of polymer networks with various surface physicochemical properties and utilized to study cell-material interaction. Extensive NMR and HPLC analyses confirmed the chemical structure of hPEG. The hydrophilicity of the polymer network was predominantly dependent on the hPEG concentration with the molecular weight and terminal functional group playing lesser roles. Adherent human fibroblast density on the network remained constant with increasing hPEG concentration in the network formulation but decreased rapidly on networks containing 0.8-1.25 g ml(-1) of hPEG. This trend was independent of the hPEG terminal moiety and molecular weight. No adherent cell was observed on all films containing 2.5 g ml(-1) or more of hPEG. |
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Authors:
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W J Kao; D Lok; J Li |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of biomaterials science. Polymer edition Volume: 12 ISSN: 0920-5063 ISO Abbreviation: J Biomater Sci Polym Ed Publication Date: 2001 |
Date Detail:
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Created Date: 2001-09-14 Completed Date: 2002-01-31 Revised Date: 2008-02-20 |
Medline Journal Info:
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Nlm Unique ID: 9007393 Medline TA: J Biomater Sci Polym Ed Country: Netherlands |
Other Details:
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Languages: eng Pagination: 599-611 Citation Subset: IM |
Affiliation:
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Division of Pharmaceutical Sciences of the School of Pharmacy, University of Wisconsin Madison, 53706, USA. wjkao@pharmacy.wisc.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biocompatible Materials
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chemistry Cell Adhesion Cell Culture Techniques / methods Cells, Cultured Chromatography, High Pressure Liquid Dose-Response Relationship, Drug Fibroblasts / metabolism Humans Magnetic Resonance Spectroscopy Models, Chemical Polyethylene Glycols / chemistry* Polymers / chemistry Skin / cytology Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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HL-63686/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Polyethylene Glycols; 0/Polymers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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