Document Detail

Preparation of a fluorescent antibody specific for the yeast phase of Histoplasma capsulatum.
MedLine Citation:
PMID:  14454457     Owner:  NLM     Status:  MEDLINE    
Kaufman, Leo (Communicable Disease Center, Atlanta, Ga.) and William Kaplan. Preparation of a fluorescent antibody specific for the yeast phase of Histoplasma capsulatum. J. Bacteriol. 82:729-735. 1961.-Rabbit anti-Histoplasma capsulatum globulin was conjugated with fluorescein isothiocyanate. This labeled antibody, in addition to staining six strains of H. capsulatum, exhibited strong cross-staining with ten strains of Blastomyces dermatitidis and numerous other species of fungi. Adsorption of the labeled antiglobulin either twice with cells of B. dermatitidis or twice with cells of Coccidioides immitis and once with cells of B. dermatitidis resulted in a good staining fluorescent antibody reagent, specific for yeast phase cells of H. capsulatum. Attempts to define the specific labeled antibody by the Ouchterlony procedure were unsuccessful. The specific conjugate did not give a complement fixation titer with yeast phase cells of B. dermatitidis or with coccidioidin. It did, however, show a 1:16 complement fixation titer with homologous yeast phase cells and a weaker reaction with histoplasmin.
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  82     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1961 Nov 
Date Detail:
Created Date:  1962-12-01     Completed Date:  1998-11-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  Not Available    
Other Details:
Languages:  eng     Pagination:  729-35     Citation Subset:  OM    
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MeSH Terms
Histoplasma / immunology*
Reg. No./Substance:

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