| Preparation of fixed mouse brains for MRI. | |
| | |
MedLine Citation:
|
PMID: 22305951 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
In fixed mouse brain magnetic resonance images, a high prevalence of fixation artifacts have been observed. Of more than 1700 images of fixed brains acquired at our laboratory, fixation artifacts were present in approximately 30%. In this study, two of these artifacts are described and their causes are identified. A hyperintense rim around the brain is observed when using perfusates reconstituted from powder and delivered at a high flow rate. It is proposed that these perfusion conditions cause blockage of the capillary beds and an increase in pressure that ruptures the vessels, resulting in a blister of liquid below the dura mater. Secondly, gray-white matter contrast inversion is observed when too short a fixation time or too low a concentration of fixative is used, resulting in inadequate fixation. The deleterious consequences of these artifacts for quantitative data analysis are discussed, and precautions for their prevention are provided. |
| | |
Authors:
|
Lindsay S Cahill; Christine L Laliberté; Jacob Ellegood; Shoshana Spring; Jacqueline A Gleave; Matthijs C van Eede; Jason P Lerch; R Mark Henkelman |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-28 |
Journal Detail:
|
Title: NeuroImage Volume: - ISSN: 1095-9572 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-2-6 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9215515 Medline TA: Neuroimage Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012 Elsevier Inc. All rights reserved. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: MRI atrophy of the caudate nucleus and slower walking speed in the elderly.
Next Document: Nonlinear denoising and analysis of neuroimages with kernel principal component analysis and pre-ima...