Document Detail

Preparation and evaluation of microporous organogel scaffolds for cell viability and proliferation.
MedLine Citation:
PMID:  20427161     Owner:  NLM     Status:  MEDLINE    
Various porous scaffolds utilizing an organogel were prepared by particulate-leaching method. The porous organogels were made of biodegradable, non-toxic ingredients like soybean oil or caprylic/capric triglyceride as the organic liquids and 12-hydroxystearic acid as the gelator. The scaffolds possessed an effective porosity of 56-65%, and good pore interconnectivity with an average pore size from 220 to 290mum. The biodegradability of such materials was evaluated and lipases were able to totally degrade the scaffolds. The porosity of the material associated with high draining led to suitable scaffolds which were evaluated for CHO cell viability and proliferation using the MTT test. This evaluation was performed over a period of 3 weeks and showed a greater ability to promote cell proliferation for the soybean oil based scaffold than for the caprylic/capric triglyceride one. The histological investigations revealed that this scaffold was able to promote cell colonization and attachment and could induce the production of collagen.
Lyubov Lukyanova; Sophie Franceschi-Messant; Patricia Vicendo; Emile Perez; Isabelle Rico-Lattes; Richard Weinkamer
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-08
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  79     ISSN:  1873-4367     ISO Abbreviation:  Colloids Surf B Biointerfaces     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-05-24     Completed Date:  2010-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  105-12     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Laboratoire des I.M.R.C.P. UMR 5623 CNRS, Université Paul Sabatier, Toulouse, France.
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MeSH Terms
Biocompatible Materials / chemistry*,  pharmacology*
CHO Cells
Cell Proliferation / drug effects*
Cell Survival / drug effects
Microscopy, Electron, Scanning
Nanostructures / chemistry,  ultrastructure
Soybean Oil / chemistry
Tissue Engineering / methods
Tissue Scaffolds / chemistry*
Reg. No./Substance:
0/Biocompatible Materials; 8001-22-7/Soybean Oil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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