| Preparation and evaluation of microparticles from thiolated polymers via air jet milling. | |
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MedLine Citation:
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PMID: 18294828 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Microparticles were formulated by incorporation of the model protein horseradish peroxidase in (thiolated) chitosan and (thiolated) poly(acrylic acid) via co-precipitation. Dried protein/polymer complexes were ground with an air jet mill and resulting particles were evaluated regarding size distribution, shape, zeta potential, drug load, protein activity, release pattern, swelling behaviour and cytotoxicity. The mean particle size distribution was 0.5-12 microm. Non-porous microparticles with a smooth surface were prepared. Microparticles from (thiolated) chitosan had a positive charge whereas microparticles from (thiolated) poly(acrylic acid) were negatively charged. The maximum protein load for microparticles based on chitosan, chitosan-glutathione (Ch-GSH), poly(acrylic acid) (PAA) and for poly(acrylic acid)-glutathione (PAA-GSH) was 7+/-1%, 11+/-2%, 4+/-0.2% and 7+/-2%, respectively. The release profile of all microparticles followed a first order release kinetic. Chitosan (0.5mg), Ch-GSH, PAA and PAA-GSH particles showed a 31.4-, 13.8-, 54.2- and a 42.2-fold increase in weight, respectively. No significant cytotoxicity could be found. Thiolated microparticles prepared by jet milling technique were shown to be stable and to have controlled drug release characteristics. After further optimizations the preparation method described here might be a useful tool for the production of protein loaded drug delivery systems. |
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Authors:
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Herbert Hoyer; Wolfgang Schlocker; Kafedjiiski Krum; Andreas Bernkop-Schnürch |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-01-19 |
Journal Detail:
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Title: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V Volume: 69 ISSN: 0939-6411 ISO Abbreviation: Eur J Pharm Biopharm Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-05-19 Completed Date: 2008-08-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9109778 Medline TA: Eur J Pharm Biopharm Country: Netherlands |
Other Details:
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Languages: eng Pagination: 476-85 Citation Subset: IM |
Affiliation:
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Leopold-Franzens-University Innsbruck, Innsbruck, Austria. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acrylic Resins
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chemistry Caco-2 Cells Cell Survival / drug effects Chitosan Drug Carriers Drug Compounding Glutathione / chemistry Horseradish Peroxidase / chemistry Humans L-Lactate Dehydrogenase / metabolism Microscopy, Electron, Scanning Microspheres Nanoparticles / chemistry* Particle Size Polymers / chemistry* Sulfhydryl Compounds / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Acrylic Resins; 0/Drug Carriers; 0/Polymers; 0/Sulfhydryl Compounds; 70-18-8/Glutathione; 9003-01-4/carbopol 940; 9012-76-4/Chitosan; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.11.1.-/Horseradish Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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