| Preparation of connexin43-integrated giant Liposomes by a baculovirus expression-liposome fusion method. | |
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MedLine Citation:
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PMID: 20589840 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Connexin-43 (Cx43) containing giant liposomes (GL) were prepared by a baculovirus expression-liposome fusion method. Recombinant budded viruses expressing Cx43 were prepared and then fused with GLs containing DOPG/DOPC at pH 4.5. Connexon formation on the GL membrane was observed by transmission electron microscope. Hydrophilic fluorescent dye transfers were observed through a Cx43-mediated pathway not only between Sf9 (Spodoptera frugiperda) cells with Cx43 but also from giant Cx43 liposomes to Cx43-expressing U2OS cells (human osteosarcoma cell). The functional connexin-containing liposome is expected to be useful for cellular cytosolic delivery systems. The original orientation and function of Cx43 was maintained after integration into the liposomes. The liposome fusion method will create new opportunities as a tool for analysis of channel membrane proteins. |
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Authors:
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Koki Kamiya; Kanta Tsumoto; Satoko Arakawa; Shigeomi Shimizu; Ikuo Morita; Tetsuro Yoshimura; Kazunari Akiyoshi |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 107 ISSN: 1097-0290 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-10-25 Completed Date: 2011-01-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 836-43 Citation Subset: IM |
Copyright Information:
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© 2010 Wiley Periodicals, Inc. |
Affiliation:
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Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Baculoviridae / genetics, growth & development* Biotechnology / methods* Cell Line Connexin 43 / genetics, metabolism* Fluorescent Dyes / metabolism Genetic Vectors* Humans Liposomes / metabolism* Recombinant Proteins / genetics, metabolism Spodoptera Staining and Labeling / methods Virion / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Connexin 43; 0/Fluorescent Dyes; 0/Liposomes; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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