Document Detail


Preparation of chylomicrons and VLDL with monoacid-rich triacylglycerol and characterization of kinetic parameters in lipoprotein lipase-mediated hydrolysis in chickens.
MedLine Citation:
PMID:  9915888     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To identify the substrate specificity of lipoprotein lipase (LPL) for triacylglycerol-rich lipoproteins with monoacid-rich triacylglycerols, monoacid-rich lipoproteins were prepared and kinetic parameters of LPL were characterized. Male broiler chickens were fed 8 g/100 g fat diets differing only in the fat source: palm oil (tripalmitin-rich), olive oil (triolein-rich), safflower oil (trilinolein-rich) and linseed oil (trilinolenin-rich). After diets were fed for 3 d, chickens were starved for 2 d and then force-fed emulsions containing one of the monoacid-triacylglycerols: tripalmitin, triolein, trilinolein or trilinolenin. The triacylglycerols in chylomicrons and very low density lipoprotein (VLDL) of chickens force-fed tripalmitin, triolein or trilinolein contained the corresponding acid at more than 70% of total acids. Linolenic acid was incorporated into chylomicrons and VLDL to a lower extent (51.2 and 57.2%, respectively) in chickens force-fed trilinolein. Major apolipoproteins and lipid compositions were not significantly different among all lipoproteins isolated from chickens fed the different fats. Vmax of LPL was significantly higher (P < 0.05) for palmitic acid-rich chylomicrons and VLDL and decreased with increasing chain length and unsaturation of monoacid: 16:0>18:1>18:2>18:3. The electron spin resonance analysis, order parameter (S), decreased with monoacid chain length and unsaturation. In addition, the Vmax of LPL increased linearly (P < 0.01, r = 0. 912) with an increase in the palmitic acid content of the lipoprotein triacylglycerols. These findings suggest that lipoprotein catalysis by LPL is modulated by the palmitic acid content of the lipoprotein triacylglycerol, which affects the fluidity of lipoproteins.
Authors:
K Sato; T Takahashi; Y Takahashi; H Shiono; N Katoh; Y Akiba
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of nutrition     Volume:  129     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-02-23     Completed Date:  1999-02-23     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  126-31     Citation Subset:  IM    
Affiliation:
Department of Animal Science, Faculty of Agriculture, Tohoku University, Sendai-shi 981, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apolipoproteins / metabolism
Chickens
Chylomicrons / metabolism*
Electron Spin Resonance Spectroscopy
Fatty Acids / metabolism*
Hydrolysis
Kinetics
Lipid Metabolism
Lipoprotein Lipase / metabolism*
Lipoproteins, VLDL / metabolism*
Male
Phospholipids / metabolism
Substrate Specificity
Triglycerides / chemistry,  metabolism*
Chemical
Reg. No./Substance:
0/Apolipoproteins; 0/Chylomicrons; 0/Fatty Acids; 0/Lipoproteins, VLDL; 0/Phospholipids; 0/Triglycerides; EC 3.1.1.34/Lipoprotein Lipase

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