Document Detail


Preparation and characterization of polypeptide-stabilized gold nanoparticles.
MedLine Citation:
PMID:  17025065     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Helical polypeptides have a highly rigid conformation. In order to control self-assembling structure in nanoscale, the rigidity of the stabilizing molecules, which is working as scaffolds, is highly important. Furthermore, the molecular lengths of polypeptides can be readily controlled by using their unique polymerization methods. In this study, we have used helical polypeptides as the stabilizing reagent of metal nanoparticles. As for rigid helical polypeptides, poly(gamma-benzyl-L-glutamate) (PBLG) was selected, and was synthesized by N-carboxylic acid anhydride (NCA) polymerization. Preparation of helical polypeptide-stabilized gold nanoparticles using a two-phase reduction process from the corresponding metal salts with NaBH4 is therefore introduced. PBLG itself did not have good stabilizing ability for metal nanoparticles and a terminal attaching ligand was indispensable for nanoparticle stabilization and, for this purpose, lipoic acid was selected. It is estimated that lipoic acid functionalized polypeptide molecules were attached perpendicularly to the particle surface by lipoic acid group. The size and structure, as well as assembling will be discussed in this manuscript.
Authors:
Tetsu Yonezawa; Takashi Nomura; Takatoshi Kinoshita; Kunihito Koumoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of nanoscience and nanotechnology     Volume:  6     ISSN:  1533-4880     ISO Abbreviation:  J Nanosci Nanotechnol     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-10-09     Completed Date:  2006-11-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101088195     Medline TA:  J Nanosci Nanotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1649-54     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
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MeSH Terms
Descriptor/Qualifier:
Drug Stability
Gold / chemistry*
Macromolecular Substances
Microscopy, Electron
Models, Molecular
Nanostructures / chemistry*,  ultrastructure
Nanotechnology / methods
Particle Size
Peptides / chemistry*
Polyglutamic Acid / analogs & derivatives,  chemistry
Protein Structure, Secondary
Thioctic Acid / chemistry
Chemical
Reg. No./Substance:
0/Macromolecular Substances; 0/Peptides; 25014-27-1/poly-gamma-benzyl-L-glutamate; 25513-46-6/Polyglutamic Acid; 62-46-4/Thioctic Acid; 7440-57-5/Gold

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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