| Preparation and characterization of paramagnetic polychelates and their protein conjugates. | |
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MedLine Citation:
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PMID: 2095206 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The gadolinium complexes of poly-L-lysine-poly(diethylenetriamine-N,N,N',N",N"-pentaacetic acid) (Gd-PL-DTPA) and poly-L-lysine-poly(1,4,7,10-tetraazacyclododecane-N,N',N",N"'-tetr aacetic acid) (Gd-PL-DOTA) and their conjugates with human serum albumin (HSA) have been prepared and characterized. Poly-L-lysine (PL, degree of polymerization approximately 100) was N-acylated with a mixed anhydride of the chelating ligand (DTPA or DOTA). Sixty to ninety chelating groups per molecule of PL could be attached in this way. Following purification of the polychelate by size-exclusion chromatography, the gadolinium complexes were prepared by standard methods and conjugated to HSA with heterobifunctional cross-linking reagents. The molar relaxities of these macromolecular species were 2-3-fold higher than those of the corresponding monomeric metal complexes [( Gd(DTPA)] and [Gd(DOTA)]). The conjugation conditions were optimized to produce conjugates containing 60-90 metal centers per molecule of HSA (ca. one polychelate per protein). |
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Authors:
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P F Sieving; A D Watson; S M Rocklage |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bioconjugate chemistry Volume: 1 ISSN: 1043-1802 ISO Abbreviation: Bioconjug. Chem. Publication Date: 1990 Jan-Feb |
Date Detail:
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Created Date: 1991-07-09 Completed Date: 1991-07-09 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9010319 Medline TA: Bioconjug Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 65-71 Citation Subset: IM |
Affiliation:
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Salutar, Incorporated, Sunnyvale, California 94086. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chelating Agents* Gadolinium* / chemistry* Gadolinium DTPA* Heterocyclic Compounds / chemical synthesis, chemistry* Humans Indicators and Reagents Ligands Organometallic Compounds / chemical synthesis, chemistry* Pentetic Acid / chemical synthesis, chemistry* Polylysine* / chemical synthesis, chemistry* Protein Binding Serum Albumin* |
| Chemical | |
Reg. No./Substance:
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0/Chelating Agents; 0/Heterocyclic Compounds; 0/Indicators and Reagents; 0/Ligands; 0/Organometallic Compounds; 0/Serum Albumin; 0/polylysine-(Gd-DOTA); 0/polylysine-(Gd-DTPA); 25104-18-1/Polylysine; 67-43-6/Pentetic Acid; 7440-54-2/Gadolinium; 80529-93-7/Gadolinium DTPA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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