Document Detail


Preparation and characterization of monodisperse core-shell Fe3O4@SiO2 microspheres and its application for magnetic separation of nucleic acids from E. coli BL21.
MedLine Citation:
PMID:  23030008     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
In this article, we present an easy route to prepare monodisperse core-shell Fe3O4@SiO2 microspheres with uniform size and shape. Their structures and properties were studied by transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectrometry (FT-IR), and vibrating sample magnetometer (VSM), respectively. The results showed that spherical Fe3O4 microspheres with well dispersion have a rough surface and an average diameter (about 500 nm). After the modification with silica, the particles have a well-defined core-shell structure and a much smoother surface and larger particle diameter (about 600 nm). Furthermore, VSM measurements indicated that the as-prepared Fe3O4 and Fe3O4@SiO2 microspheres were superparamagnetic at room temperature and the saturation magnetization (M(s)) were 58.110 emu/g and 33.479 emu/g, respectively. And then, the prepared monodisperse core-shell Fe3O4@SiO2 microspheres were subsequently applied to separate nucleic acids from the bacteria (E. coli BL21) and verified the great application prospects for bioseparation technology of the biomoleculars.
Authors:
Chao Ma; Chuanyan Li; Nongyue He; Fang Wang; Ningning Ma; Liming Zhang; Zhuoxuan Lu; Zeeshan Ali; Zhijiang Xi; Xiaolong Li; Gaofeng Liang; Hongna Liu; Yan Deng; Lijian Xu; Zhifei Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical nanotechnology     Volume:  8     ISSN:  1550-7033     ISO Abbreviation:  J Biomed Nanotechnol     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-10-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101230869     Medline TA:  J Biomed Nanotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1000-5     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Nanjing 210096, PR China.
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