| Preparation and characterization of magnetic nanoparticles containing Fe(3)O(4)-dextran-anti-β-human chorionic gonadotropin, a new generation choriocarcinoma-specific gene vector. | |
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MedLine Citation:
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PMID: 21383853 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To evaluate the feasibility of using magnetic iron oxide (Fe(3)O(4))-dextran-anti-β-human chorionic gonadotropin (HCG) nanoparticles as a gene vector for cellular transfections. STUDY DESIGN: Fe(3)O(4)-dextran-anti-β-HCG nanoparticles were synthesized by chemical coprecipitation. The configuration, diameter, and iron content of the nanoparticles were detected by transmission electron microscopy (TEM), light scatter, and atomic absorption spectrophotometry. A3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay was used to evaluate the cytotoxicity of Fe(3)O(4)-dextran-anti-β-HCG nanoparticles. Enzyme-linked immunosorbent assay and indirect immunofluorescence were used to evaluate immunoreactivity. The efficiency of absorbing DNA and resisting deoxyribonuclease I (DNase I) digestion when bound to Fe(3)O(4)-dextran-anti-β-HCG nanoparticles was examined by agarose gel electrophoresis. The ability of Fe(3)O(4)-dextran-anti-β-HCG nanoparticles to absorb heparanase antisense oligodeoxynucleotides (AS-ODN) nanoparticles in different cell lines was evaluated by flow cytometry. The tissue distribution of heparanase AS-ODN magnetic nanoparticles in choriocarcinoma tumors transplanted in nude mice was detected by atomic absorption spectrophotometry. RESULTS: TEM demonstrated that the shape of nanoparticles is irregular. Light scatter revealed nanoparticles with a mean diameter of 75.5 nm and an iron content of 37.5 μg/mL. No cytotoxicity was observed when the concentration of Fe(3)O(4)-dextran-anti-β-HCG nanoparticles was <37.5 μg/mL. Fe(3)O(4)-dextran nanoparticles have a satisfactory potential to combine with β-HCG antibody. Agarose gel electrophoresis analysis of binding experiments showed that after treatment with sodium periodate, Fe(3)O(4)-dextran-anti-β-HCG nanoparticles have a satisfactory potential to absorb DNA, and the protection experiment showed that nanoparticles can effectively protect DNA from DNase I digestion. Aldehyde Fe(3)O(4)-dextran-anti-β-HCG nanoparticles can transfect reporter genes, and the transfection efficiency of these nanoparticles is greater than that of liposomes (P < 0.05). Fe(3)O(4)-dextran-anti-β-HCG nanoparticles can concentrate in choriocarcinoma cells and in transplanted choriocarcinoma tumors. CONCLUSIONS: The results confirm that Fe(3)O(4)-dextran-anti-β-HCG nanoparticles have potential as a secure, effective, and choriocarcinoma-specific targeting gene vector. |
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Authors:
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Cai Jingting; Liu Huining; Zhang Yi |
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Publication Detail:
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Type: Journal Article Date: 2011-02-02 |
Journal Detail:
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Title: International journal of nanomedicine Volume: 6 ISSN: 1178-2013 ISO Abbreviation: Int J Nanomedicine Publication Date: 2011 |
Date Detail:
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Created Date: 2011-03-08 Completed Date: 2011-06-24 Revised Date: 2013-05-29 |
Medline Journal Info:
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Nlm Unique ID: 101263847 Medline TA: Int J Nanomedicine Country: New Zealand |
Other Details:
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Languages: eng Pagination: 285-94 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China. cjingting114@yahoo.com.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / administration & dosage, chemistry*, immunology Cell Line, Tumor Choriocarcinoma / genetics Chorionic Gonadotropin / antagonists & inhibitors*, immunology DNA / chemistry, metabolism Deoxyribonuclease I / chemistry, metabolism Dextrans / administration & dosage, chemistry, pharmacokinetics Enzyme-Linked Immunosorbent Assay Female Ferric Compounds / administration & dosage, chemistry*, pharmacokinetics Flow Cytometry Fluorescent Antibody Technique, Indirect Genetic Vectors / administration & dosage, chemistry*, genetics HeLa Cells Humans Magnetite Nanoparticles / administration & dosage, chemistry* Mice Mice, Inbred BALB C Mice, Nude Microscopy, Electron, Transmission |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Chorionic Gonadotropin; 0/Ferric Compounds; 0/Magnetite Nanoparticles; 1309-37-1/ferric oxide; 9004-54-0/Dextrans; 9007-49-2/DNA; EC 3.1.21.1/Deoxyribonuclease I |
| Comments/Corrections | |
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