| Preparation, characterization, and in vivo evaluation of insulin-loaded PLA-PEG microspheres for controlled parenteral drug delivery. | |
| | |
MedLine Citation:
|
PMID: 19832637 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
AIM: The aim of this study was to prepare insulin-loaded poly(lactic acid)-polyethylene glycol microspheres that could control insulin release at least for 1 week and evaluate their in vivo performance in a streptozotocin-induced diabetic rat model. METHODS: The microspheres were prepared using a water-in-oil-in-water double emulsion solvent evaporation technique. Different formulation variables influencing the yield, particle size, entrapment efficiency, and in vitro release profiles were investigated. The pharmacokinetic study of optimized formulation was performed with single dose in comparison with multiple dose of Humulin 30/70 as a reference product in streptozotocin-induced diabetic rats. RESULTS: The optimized formulation of insulin microspheres was nonporous, smooth-surfaced, and spherical in structure under scanning electron microscope with a mean particle size of 3.07 microm and entrapment efficiency of 42.74% of the theoretical amount incorporated. The in vitro insulin release profiles was characterized by a bimodal behavior with an initial burst release because of the insulin adsorbed on the microsphere surface, followed by slower and continuous release corresponding to the insulin entrapped in polymer matrix. CONCLUSIONS: The optimized formulation and reference were comparable in the extent of absorption. Consequently, these microspheres can be proposed as new controlled parenteral delivery system. |
| | |
Authors:
|
Ravi Sheshala; Kok Khiang Peh; Yusrida Darwis |
Related Documents
:
|
10326827 - Role of insulin preincubation in the contractile reactivity of rat aortic rings. 1701127 - Modulation of glucose-induced insulin secretion from a rat clonal beta-cell line. 16826597 - The mathematical model of insulin desorption from the bioactive, fibrous artificial store. 7681407 - Blockade of galanin-induced inhibition of insulin secretion from isolated mouse islets ... 12560587 - Insulin sensitizers and atherosclerosis. 468377 - Phosphoenolpyruvate-dependent sucrose phosphotransferase activity in streptococcus muta... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Drug development and industrial pharmacy Volume: 35 ISSN: 1520-5762 ISO Abbreviation: Drug Dev Ind Pharm Publication Date: 2009 Nov |
Date Detail:
|
Created Date: 2009-10-16 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7802620 Medline TA: Drug Dev Ind Pharm Country: England |
Other Details:
|
Languages: eng Pagination: 1364-74 Citation Subset: IM |
Affiliation:
|
School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang, Malaysia. ravigdk2002@yahoo.co.uk |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Why does a hydrophilic drug permeate skin, although it is not soluble in white petrolatum?
Next Document: Modified nanoprecipitation method to fabricate DNA-loaded PLGA nanoparticles.