| Preparation and characterization of camptothecin solid lipid nanoparticles. | |
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MedLine Citation:
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PMID: 12026219 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Camptothecin (CA), an antitumor drug, was incorporated into solid lipid nanoparticles (SLNs) prepared by high-pressure homogenization. A Taguchi orthogonal experimental design was used to study the influence of four different variables, with each variable having three value levels on nanoparticle size. Analysis of variance (ANOVA) has been used to evaluate the preparation of CA-SLNs and perform product optimization. The optimized CA-SLNs suspension was lyophilized using mannitol and glucose as cryoprotectants. The physicochemical characteristics of CA-SLNs were evaluated using transmission electron microscopy (TEM), electrophoresis, and differential scanning calorimetry (DSC). The release of camptothecin from CA-SLNs in various media was evaluated using a high-performance liquid chromatography (HPLC) method. The results showed that the concentration of emulsifier and the homogenization pressure had a significant influence on the particle size. The optimized CA-SLNs had an average diameter of about 200 nm, exhibited monodispersity with Dw/Dn of 1.06, and carried a negative charge. The optimal cryoprotectants consisted of 10% mannitol and 5% glucose in nanoparticle suspension. Lyophilized product was reconstituted in distilled water within 0.5 min without change of nanoparticle size. Camptothecin might exist in an amorphous state in SLNs. In vitro results showed that drug release was achieved for up to one week, and the released camptothecin quickly changed to open carboxylate form in the biological pH phosphate buffer. The results indicate that SLNs might be good potential sustained-release delivery vehicles for camptothecin or other lipophilic drugs. |
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Authors:
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Shi Cheng Yang; Jia Bi Zhu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Drug development and industrial pharmacy Volume: 28 ISSN: 0363-9045 ISO Abbreviation: Drug Dev Ind Pharm Publication Date: 2002 Mar |
Date Detail:
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Created Date: 2002-05-23 Completed Date: 2002-12-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7802620 Medline TA: Drug Dev Ind Pharm Country: United States |
Other Details:
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Languages: eng Pagination: 265-74 Citation Subset: IM |
Affiliation:
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Zhongkun Pharmaceutical Research Institute, China Pharmaceutical University, Nanjing, Jiangsu, People's Republic of China. yangshicheng@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Camptothecin
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chemical synthesis,
chemistry*,
pharmacokinetics Capsules Nanotechnology / methods*, statistics & numerical data |
| Chemical | |
Reg. No./Substance:
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0/Capsules; 7689-03-4/Camptothecin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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