Document Detail


Preparation and characterization of alginate microspheres for sustained protein delivery within tissue scaffolds.
MedLine Citation:
PMID:  23302146     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Tissue engineering scaffolds are designed not only to provide structural support for the repair of damaged tissue, but can also serve the function of bioactive protein delivery. Here we present a study on the preparation and characterization of protein-loaded microspheres, either alone or incorporated into mock tissue scaffolds, for sustained protein delivery. Alginate microspheres were prepared by a novel, small-scale water-in-oil emulsion technique and loaded with fluorescently labeled immunoglobulin G (IgG). Microsphere size appears to be influenced by the magnitude and distribution of force generated by mechanical stirring during emulsion. Protein release studies show that sustained IgG release from microspheres could be achieved and that application of a secondary coating of chitosan could further slow the rate of protein release. Preservation of bioactivity of released IgG protein was confirmed using an immunohistochemical assay. When IgG-loaded microspheres were incorporated into mock scaffolds, initial protein release was diminished and the overall time course of release was extended. The present study demonstrates that protein-loaded microspheres can be prepared with a controlled release profile and preserved biological activity, and can be incorporated into scaffolds to achieve sustained and prolonged protein delivery in a tissue engineering application.
Authors:
Peng Zhai; X B Chen; David J Schreyer
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-10
Journal Detail:
Title:  Biofabrication     Volume:  5     ISSN:  1758-5090     ISO Abbreviation:  Biofabrication     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101521964     Medline TA:  Biofabrication     Country:  -    
Other Details:
Languages:  ENG     Pagination:  015009     Citation Subset:  -    
Affiliation:
Division of Biomedical Engineering, University of Saskatchewan, Saskatchewan S7N5A9, Canada.
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