Document Detail

Preparation and characterization of alginate microspheres for sustained protein delivery within tissue scaffolds.
MedLine Citation:
PMID:  23302146     Owner:  NLM     Status:  Publisher    
Tissue engineering scaffolds are designed not only to provide structural support for the repair of damaged tissue, but can also serve the function of bioactive protein delivery. Here we present a study on the preparation and characterization of protein-loaded microspheres, either alone or incorporated into mock tissue scaffolds, for sustained protein delivery. Alginate microspheres were prepared by a novel, small-scale water-in-oil emulsion technique and loaded with fluorescently labeled immunoglobulin G (IgG). Microsphere size appears to be influenced by the magnitude and distribution of force generated by mechanical stirring during emulsion. Protein release studies show that sustained IgG release from microspheres could be achieved and that application of a secondary coating of chitosan could further slow the rate of protein release. Preservation of bioactivity of released IgG protein was confirmed using an immunohistochemical assay. When IgG-loaded microspheres were incorporated into mock scaffolds, initial protein release was diminished and the overall time course of release was extended. The present study demonstrates that protein-loaded microspheres can be prepared with a controlled release profile and preserved biological activity, and can be incorporated into scaffolds to achieve sustained and prolonged protein delivery in a tissue engineering application.
Peng Zhai; X B Chen; David J Schreyer
Related Documents :
23650106 - Protein resistance of surfaces modified with oligo(ethylene glycol) aryl diazonium deri...
24870016 - Ultra-high-throughput screening of natural product extracts to identify proapoptotic in...
24850806 - Kinetic assay of the michael addition-like thiol-ene reaction and insight into protein ...
24401286 - Prenylation defects in inherited retinal diseases.
25348176 - Tf-independent inhibition of thrombin generation by tfpia.
12560556 - Nuclear matrix localization and sumo-1 modification of adenovirus type 5 e1b 55k protei...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-10
Journal Detail:
Title:  Biofabrication     Volume:  5     ISSN:  1758-5090     ISO Abbreviation:  Biofabrication     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101521964     Medline TA:  Biofabrication     Country:  -    
Other Details:
Languages:  ENG     Pagination:  015009     Citation Subset:  -    
Division of Biomedical Engineering, University of Saskatchewan, Saskatchewan S7N5A9, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The First-aid Advice and Safety Training (FAST) parents programme for the prevention of unintentiona...
Next Document:  Development and validation of the K-VSCOR for scoring Koebner's phenomenon in Vitiligo/non-segmental...